| 西藏高寒植物垫状雪灵芝酯酶同工酶变异研究 |
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| 引用本文： | 兰小中,权红,郑维列. 西藏高寒植物垫状雪灵芝酯酶同工酶变异研究[J]. 干旱区资源与环境, 2009, 23(1): 187-192 |
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| 作者姓名： | 兰小中 权红 郑维列 |
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| 作者单位： | 西藏大学农牧学院动物科学院,林芝,860000;西藏大学农牧学院高原生态研究所,林芝,860000 |
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| 基金项目： | 973计划前期研究专项(项目名称:西藏高寒植物种群生存机制研究;编号:2006CB708403)资助 |
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| 摘 要： | 以色季拉山的高寒植物垫状雪灵芝为材料,选取分布在海拔4500~4650m范围内的垫状雪灵芝3个居群为研究对象,采用聚丙烯酰胺琼脂电泳技术,对垫状雪灵芝3个居群的48个个体功能叶片的酯酶同工酶谱带进行测定并编码,依据酶谱带测定结果进行聚类分析;同时结合酯酶同工酶的3个遗传指标进行相关分析。结果表明:来自同一居群的个体,同工酶谱带相似性较大,居群内的遗传杂合度较小(HeA=0.735,HeB=0.765,HeC=0.726),这也说明居群内个体存在一定程度的遗传分化;酯酶在不同居群间的相似性系数的平均值相对较小,遗传距离相对较大(IAB=0.392,IAC=0.542,IBC=0.610;DAB=0.937,DAC=0.613,DBC=0.495)。研究数据显示垫状雪灵芝酯酶同工酶在不同的居群内与居群间存在不同程度的遗传分化,缺少有效的基因交流。
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| 关 键 词： | 垫状雪灵芝 同工酶 酯酶 遗传多样性 西藏 |
Study on Variance of Esterase Isozymic of Alpine Plant Arenaria pulvinata Edgew.in Tibet |
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| LAN Xiao-zhong,QUAN Hong,ZHENG Wei-lie. Study on Variance of Esterase Isozymic of Alpine Plant Arenaria pulvinata Edgew.in Tibet[J]. Journal of Arid Land Resources and Environment, 2009, 23(1): 187-192 |
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| Authors: | LAN Xiao-zhong QUAN Hong ZHENG Wei-lie |
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| Affiliation: | 1.Department of the Animal Science and Technology;Agriculture and Animal Husbandry College;Tibet University;Linzhi Tibet 860000;2.Research Institute of Plateau Ecology;Linzhi 860000;China |
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| Abstract: | The Esterase(EST) isozymic bands of functional leaves of 48 individual from 3 isolated population of alpine plant Arenaria pulvinata Edgew.were determined by polyacrylamide gel electrophoresis and coded,which developed on altitude 4500~4650m range in Sejila Mount.Meanwhile the cluster analysis of the isozymic bands was made and 3 genetic indexes of esterase were analysed.The results indicated that identity index of individuals from the same population is big according to the isozymic bands,genetic heterozyg... |
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| Keywords: | Arenaria pulvinata Edgew. Isozymes esterase genetic diversity Tibet |
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