| 四川‘竹根姜’胚性细胞悬浮系的建立与植株再生 |
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| 引用本文： | 郭英华,关秋竹,张振贤.四川‘竹根姜’胚性细胞悬浮系的建立与植株再生[J].园艺学报,2005,32(5):905-907. |
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| 作者姓名： | 郭英华 关秋竹 张振贤 |
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| 作者单位： | 中国农业大学农学与生物技术学院蔬菜系,北京,100094 |
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| 基金项目： | 国家科技攻关项目(2001BA511B-08-12)，农业部农业结构调整项目(04-06-02B) |
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| 摘 要： | 选用四川省地方品种‘竹根姜’的茎尖组织建立了体细胞胚性细胞悬浮系, 并通过悬浮培养获得了姜再生植株。结果表明悬浮细胞干质量受pH影响。接种量和AgNO3 对悬浮细胞的生长都有影响:最佳接种量为1.0% , 最适AgNO3浓度为6.0 mg·L - 1。胚性细胞悬浮系增殖后, 接种到前期筛选的成熟分化培养基上获得了再生植株。
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| 关 键 词： | 姜 悬浮系 体细胞胚 植株再生 |
| 文章编号： | 0513-353X（2005）05-0905-03 |
| 收稿时间： | 2005-01-03 |
| 修稿时间： | 2005-01-032005-08-13 |
Establishment and Plant Regeneration of Somatic Embryogenic Cell Suspension Cultures in Ginger(Zingiber officinale Rosc.) |
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| Guo Yinghua,Guan Qiuzhu,Zhang Zhenxian.Establishment and Plant Regeneration of Somatic Embryogenic Cell Suspension Cultures in Ginger(Zingiber officinale Rosc.)[J].Acta Horticulturae Sinica,2005,32(5):905-907. |
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| Authors: | Guo Yinghua Guan Qiuzhu Zhang Zhenxian |
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| Institution: | Guo Yinghua,Guan Qiuzhu,and Zhang Zhenxian~* |
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| Abstract: | Somatic embryogenic suspension cultures of‘Zhugenjiang’ginger cultivars of Sichuan province were established. From which, regenerated plantlets were obtained. The relationship between the dry mass of suspension cultures and pH changes in medium was also discussed. The effects of inoculation andAgNO3 on the growth of embryogenic suspensions were also discussed. The optimum concentrations of inoculation and AgNO3 were 1.0% and 6.0 mg·L-1 , respectively. |
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| Keywords: | Ginger Suspension culture Somatic embryogenesis Plant regeneration |
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