红栀子愈伤组织诱导研究

目的]为了研究红栀子组织培养的最佳条件,为栀子黄色素的工业化生产提供理论依据。方法]以红栀子茎尖、茎段和幼嫩叶片为外植体,在MS、1/2MS和1/4MS3种培养基上进行培养试验,研究不同外植体、培养基、激素浓度和消毒时间对诱导愈伤组织的影响。结果]红栀子茎尖和幼嫩叶片的诱导效果明显优于茎段。在3种培养基中,MS培养基更有利于愈伤组织的产生和生长。在MS培养基中添加2,4-D时,2.0mg/L为诱导红栀子茎尖愈伤组织的最适浓度。用升汞消毒,茎尖、茎段和幼嫩叶片的最佳消毒时间分别为9、12和7min。结论]MS+2,4-D2.0mg/L+6-BA1.0mg/L为红栀子幼嫩叶片愈伤组织的最佳培养基,且10d后形成愈伤组织。

Study on Callus Induction of Gardenia jasminonides Ellis

Objective The research aimed to investigate the optimum conditions for the tissue culture of Gardenia jasminonides Ellis so as to provide the theoretical basis for the industrialization production of gardenia yellow pigment.Method In the callus culture experiment with the stem tips,stem segments and tender leaves of G.jasminonides as explants,3 kinds of culture medium of MS,1/2MS and 1/4MS were used to study the effects of different explants,culture medium,hormone concentrations and disinfection time on the callus induction.Result The induction effects of stem tips and tender leaves were obviously better than that of stem segments.Among 3 kinds of culture medium,MS culture medium was more favorable for the induction and growth of callus.When 2,4-D was supplemented to MS culture medium,the suitable concentration for inducing the callus of stem tips was 2.0 mg/L.When mercuric chloride was used to sterilize,the optimum sterilization time for stem tips,stem segments and tender leaves were 9,12 and 7 min respectively.Conclusion The optimum culture medium for the callus of tender leaves of G.jasminonides was MS 2,4-D 2.0 mg/L 6-BA 1.0 mg/L and the callus was formed after 10 days.