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甘蓝型油菜DH群体10个主要农艺性状的遗传分析
引用本文:黄吉祥,汪义龙,倪西源,任丽平,曹明富,赵坚义.甘蓝型油菜DH群体10个主要农艺性状的遗传分析[J].浙江农业学报,2009,21(5):0.
作者姓名:黄吉祥  汪义龙  倪西源  任丽平  曹明富  赵坚义
作者单位:1.浙江省农业科学院,作物与核技术利用研究所,浙江杭州310021;2. 扬州大学生物科学与技术学院,江苏扬州225000;3. 河南省开封高级中学,河南开封475000;4.杭州师范大学,浙江杭州310021
基金项目:国家高技术研究发展计划("863"计划)项目,浙江省科技计划项目 
摘    要:在西安与杭州两环境下,以小孢子培养产生的甘蓝型油菜SG—DH群体为材料,对油菜植株主要产量构成性状、生育特性和含油量以及蛋白质含量等性状进行遗传分析。结果显示:各性状均为多基因控制的数量性状,株高、第一分枝数、荚果长度、初花期与成熟期估计的基因对数与定位的QTL数相近,而千粒重与荚果粒数估计的基因对数少于检测到的QTL数;各性状的偏度和峰度的估算结果显示,株高、初花期、终花期基因间均表现为重叠作用。由各性状之间的偏相关分析得出:两环境中株高与荚果粒数、千粒重呈极显著正相关:3个生育期相互之间表现为极显著负相关;含油量与种子蛋白质含量呈极显著负相关。

关 键 词:甘蓝型油菜  数量性状  DH群体  遗传分析  QTL定位  

Genetic analysis of ten agronomic traits using doubled haploid population in Brassica napus L.
HUANG Ji-xiang,WANG Yi-long,NI Xi-yuan,REN Li-ping,CAO Ming-fu,ZHAO Jian-yi.Genetic analysis of ten agronomic traits using doubled haploid population in Brassica napus L.[J].Acta Agriculturae Zhejiangensis,2009,21(5):0.
Authors:HUANG Ji-xiang  WANG Yi-long  NI Xi-yuan  REN Li-ping  CAO Ming-fu  ZHAO Jian-yi
Institution:1.Insititute of Crops and Utilization of Nuclear Technology,Zhejiang Academy of Agricultural Science,Hangzhou 310021, China;2.A cademy of Life Science and Technology, Yanzhou University, Yangzhou 225000, China; 3.Kaifeng Senior High School of Henan Province ,Kaifeng 475000,China;4.Hangzhou Normal University,Hangzhou 310021, China
Abstract:A F_1 derived doubled haploid (DH) population from a cross between the German cultivar Sollux and the Chinese cultivar Gaoyou was used in this study. In total,282 DH lines were evaluated in replicated field experiments in two locations,Xi'an and Hangzhou,respectively. Ten agronomic and seed traits including yield components, developmental stages,oil and protein content ,were analysed by classical genetic statistics and compared with the QTL mapping approach. The results indicated that all the traits showed typical quantitative characteristics in nature. There was no distinct difference between the QTL number and estimated number of genes in plant height, number of primary branches,silique length,days from sowing to flower and days from sowing to maturity,while the number of QTL identified for thousand seeds weight and number of seeds per silique were much less than that obtained from classical genetic analysis. According to estimated coefficient of skewness and kurtosis, duplicate interaction was deduced from plant height,days from sowing to flower and days from sowing to the end of flower. Partial correlations among the 10 traits revealed significantly positive correlation between plant height and number of seeds per silique,and between plant height and 1000 seeds weight as well;Significantly negative correlations among the three developmental stages and between oil content and seed protein content were in agreement with previous researches.
Keywords:Brassica napus L    quantitative traits  DH population  genetic analysis  QTL mapping
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