| 乙烯受体基因LeETR1在番茄Epi和VFN8中的表达及反义表达载体构建 |
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| 引用本文： | 郑铁松,应铁进,何国庆,曾广文. 乙烯受体基因LeETR1在番茄Epi和VFN8中的表达及反义表达载体构建[J]. 浙江大学学报(农业与生命科学版), 2001, 27(2): 156-160 |
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| 作者姓名： | 郑铁松 应铁进 何国庆 曾广文 |
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| 作者单位： | 1. 浙江大学食品科学与营养系，浙江杭州 310029
2. 浙江大学生物科学系，浙江杭州 310029 |
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| 基金项目： | Supported by NationalNatural Science Foundation of China (39870512) |
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| 摘 要： | 采用RPA方法对番茄乙烯过表达单基因突变体Epi和野生型VFN8中LeETR2 mRNA的表达特征进行了研究。结果表明，在所有被检组织中LeETR2 mRNA均表达，其表达丰度在叶组织中呈发育调节模式，但不受内源乙烯含量的影响；而在果实成熟后期受乙烯的轻微诱导。LeETR2的这种表达模式明显有别于其他乙烯受体基因。为了进一步研究LeETR2的功能，构建了LeETR2反义基因表达载体系统。
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| 关 键 词： | 乙烯受体 LeETR2 反义基因 核酸酶保护分析 番茄 |
| 文章编号： | 1008-9209(2001)02-0156-05 |
| 修稿时间： | 2000-07-23 |
Expression of ethylenereceptor gene LeETR2 in tomato mutant Epi and construction of its antisense expressionvector |
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| ZHENG Tie song ,YING Tie jin ,ZENG Guang wen ,HE Guo qing. Expression of ethylenereceptor gene LeETR2 in tomato mutant Epi and construction of its antisense expressionvector[J]. Journal of Zhejiang University(Agriculture & Life Sciences), 2001, 27(2): 156-160 |
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| Authors: | ZHENG Tie song YING Tie jin ZENG Guang wen HE Guo qing |
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| Affiliation: | ZHENG Tie song 1,YING Tie jin 1,ZENG Guang wen 2,HE Guo qing 1 |
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| Abstract: | The expressive characteristics of LeETR2 mRNA in tomato mutant Epi and its wild type VFN8 have been studied with RNase protect analysis (RPA) technique. The results showed that LeETR2 mRNA expressed in all the tissues examined, and it's expression was unaffected by the endogenous ethylene in leaves. However, LeETE2 mRNA was developmentally regulated, and partially induced by ethylene in fruits. The results indicated that LeETR2 mRNA exhibits different expression pattern from other ethylene receptor genes in tomato. Moreover, LeETR2 antisense gene expression vector has been constructed for further research. |
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| Keywords: | Ethylene receptor LeETR2 Antisense gene RPA Tomato |
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