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| 五味子总RNA提取方法的建立 | |
| 引用本文： | 闫恩维,刘华,王玉成.五味子总RNA提取方法的建立[J].中国林副特产,2009(4):17-18. |
| 作者姓名： | 闫恩维 刘华 王玉成 |
| 作者单位： | 1. 黑龙江省林副特产研究所,黑龙江省非木质林产品研发重点实验室,牡丹江,157011 2. 大兴安岭松岭林业局森林经营处 3. 东北林业大学林学院 |
| 基金项目： | 黑龙江省自然科学基金项目 |
| 摘 要： | 五味子是一种多糖含量很高的木本植物，由于多糖的干扰，其RNA制备比较困难。研究以五味子的叶片为材料，建立了一种稳定的从五味子中分离总RNA的方法。该方法以CTAB为基本提取试剂，通过酚、仿抽提纯化，最后应用LiCl来沉淀RNA，可以在1h内提取出纯度高的RNA。RNA质量可以满足cDNA文库构建、RT—PCR等多种分子生物学研究要求。 |
| 关 键 词： | RNA提取 五味子 多糖 LiCl沉淀 |
A Method for Isolation of Total RNA from Schisandra chinesis |
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| Yan Enwei,Liu Hua,Wang Yucheng.A Method for Isolation of Total RNA from Schisandra chinesis[J].Forest By-product and Speciality in China,2009(4):17-18. | |
| Authors: | Yan Enwei Liu Hua Wang Yucheng |
| Institution: | 1.Heilongjiang Institute of Forest By-product and Speciality;Key laboratory of Forest By-product and Speciality;Heilongjiang Mudanjiang 157011;2.Daxinganling Forestry management Burean of Songling;3.College of Forestry;Northeast Forestry University |
| Abstract: | Schisandra chinesis is a woody plant which had high level of polysaccharide.Due to the interference of polysaccharide,it is difficult to isolate RNA from Schisandra chinesis.In the present study,a method for RNA isolation from leaves of Schisandra chinesis was build.This method ia a kind of CTAB method,using phenol and chloroform to purify RNA,and RNA was deposited by LiCl,The entire experiment can be completed in 1 h.The quantities of RNA can meet the request of many experiments,such as cDNA library constr... |
| Keywords: | RNA isolation Schisandra chinesis Polysaccharide LiCl deposition |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |