粉蕉种植土镰刀菌分离与鉴定

利用PPA培养基对粉蕉不同生长周期根际土壤中镰刀菌进行分离,通过形态学和分子生物学对分离的菌株进行描述与鉴定,并分析粉蕉不同生长周期根际土壤中镰刀菌群落变化.试验结果表明,在粉蕉不同生长期根际土壤中共分离出31株镰刀菌,经形态学和分子生物学鉴定,31株镰刀菌分属于茄病镰刀菌(Fus arium solani)、尖孢镰刀菌(Fusarium oxysporum)、木贼镰刀菌(Fusarium equiseti)、层出镰刀菌(Fusarium proliferatum)和串珠镰刀菌(Gibberella moniliformis).随着粉蕉生长,粉蕉根际土壤中可分离的镰刀菌种类表现出先上升后下降的趋势,且菌株种类逐渐稳定.在生殖生长期,由于受到枯萎病病原菌的影响,土壤中镰刀菌种类产生显著变化,菌株种类数量显著增加.

Separation and identification of fusarium in banana planted soil

In order to analyze the population variation of fusarium in banana rhizosphere soil collected in different growth cycle of banana, the fusarium was separated by PPA medium and identified by morphology and molecular methods. The results showed that 31 strains of fusarium were separated from rhizosphere soil collected in different growth cycle of banana, and were identified as Fusarium solani, Fusarium oxysporum, Fusarium equiseti, Fusarium proliferatum and Gibberella moniliformis. Along with the growth of banana, the quantity of fusarium species increased first, then decreased. At the end of vegetative growth stage of banana, variation of fusarium species tended to be stable. But at the reproductive stage of banana, due to affected by the pathogen of banana fusarium wilt, the quantity of fusarium species increased greatly.