水稻广亲和基因S5 n新功能标记的设计与验证

广亲和基因S5n可恢复籼粳杂种育性,是亚种间优势利用的重要资源.S5n已经克隆,分子标记技术成为了鉴定其是否存在的快速有效手段.根据S5n存在136 bp碱基的缺失,设计均可在聚丙烯酰胺凝和琼脂糖凝胶这两种介质上电泳检测的S5n基因功能标记InDel-S5n,其扩增片段大小为224 bp(基因S5n)或360 bp(基因S5i或S5j).通过10个常规水稻品种及1个F2群体230单株对InDel-S5n进行验证,结果表明,所设计标记均能较好地在琼脂糖凝胶和聚丙烯酰胺凝胶中进行检测,且能准确地鉴定供试材料是否包含目的基因及基因类型(纯合或杂合型),可见该标记可以用于S5n基因资源筛选、分子标记辅助选择育种.

Design and validation for a new functional marker of wide compatibility gene S5n in rice

The indica japonica hybrid sterility can be restored by wide compatibility gene S5n, which becomes an important resource to the utilization of heterosis between rice subspecies indica and japonica. The S5n gene has been cloned.Obviously, molecular marker technology has become a fast and effective means to identify the gene. According the 136 bp deletion in the DNA sequence of S5n, a new functional marker InDel -S5n was developed based on the agarose gel electrophoresis and polyacrylamide gel electrophoresis, which amplified 224 bp (gene S5n) or 360 bp (gene S5i or S5j). To validate the InDel-S5n, 10 conventional rice varieties and an F2 population of 230 plants were used, the experimental results indicated that the design marker could be detected relatively well in an agarose gel and polyacrylamide gel, and could accurately identify whether the test material comprising the gene and genotype (homozygous or heterozygous). Therefore, the InDel-S5n is practical to be used for S5n gene screening, molecular marker assisted selection breeding.