Expression of Stay-Green encoding gene (BoSGR) during postharvest senescence of broccoli |
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| Institution: | 1. Instituto de Fisiología Vegetal (INFIVE) UNLP-CONICET, 113 and 61, 1900 La Plata, Argentina;2. Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús (IIB-INTECH) UNSAM-CONICET, Av Intendente Marino Km 8.2, Chascomús B7130IWA, Buenos Aires, Argentina;3. Facultad de Ciencias Exactas, Universidad Nacional de La Plata (UNLP), 47 and 115, 1900 La Plata, Argentina;1. Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA)-CONICET, calle 47 y 116, Facultad de Ciencias Exactas, Universidad Nacional de La Plata (UNLP), 47 y 115, 1900, La Plata, Argentina;2. Laboratorio de Investigación en Productos Agroindustriales (LIPA), Facultad de Ciencias Agrarias y Forestales- UNLP, calle 60 y 119, CP 1900, La Plata, Argentina;3. Instituto de Investigaciones Biotecnológicas–Instituto Tecnológico de Chascomús (IIB–INTECH), UNSAM–CONICET, Av. Intendente Marino Km 8,5 (B7130IWA) Chascomús, Argentina;1. Graduate School of Life Science, Hokkaido University, N10 W8, Sapporo, 060-0810, Japan;2. Institute of Low Temperature Science, Hokkaido University, N19 W8, Sapporo, 060-0819, Japan;1. Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea;2. Institute of Plant Biology, University of Zurich, CH-8008 Zurich, Switzerland;1. Pontificia Universidad Católica de Valparaíso, School of Agronomy, Chile;2. Department of Postharvest Science of Fresh Produce, Volcani Center, Israel |
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| Abstract: | Degreening caused by chlorophyll degradation is the most important feature that determines postharvest loss of quality in broccoli. Chlorophyll molecules are assembled to several thylakoid proteins, from which chlorophylls must be released in order to be catabolized. Stay-Green (SGR), a chloroplast-located protein, specifically interacts with light harvesting complex subunits helping toward their destabilization and to the release of chlorophylls. In this work, a fragment of a gene encoding a SGR from broccoli (BoSGR) was cloned. The expression of BoSGR was analyzed and detected an important increment during postharvest senescence, simultaneously with chlorophyll degradation. In order to analyze the effect of different growth regulators, different groups of broccoli heads were treated with cytokinins, ethylene and 1-MCP. Cytokinins and 1-MCP delayed the increment of BoSGR expression whereas ethylene accelerated the process. In addition, several postharvest treatments that delay degreening in broccoli florets were applied to evaluate their effects on BoSGR expression. Samples treated with modified atmosphere, hot air, UV-C or white lights showed a delay in chlorophyll degradation and degreening. In most cases, the treatments also delayed the increment of BoSGR expression during senescence, reaffirming the relationship between the expression of this gene and chlorophyll degradation. |
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| Keywords: | Broccoli Senescence Chlorophyll degradation Stay-green |
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