粗皮桉再生系统的研究(英文)

该研究在国内外首次报导了粗皮桉的再生系统 .利用粗皮桉的外植体通过两种不同的再生途径建立了高效的再生系统 .首先探讨了粗皮桉的下胚轴和子叶的体细胞胚胎发生 .研究发现 ,下胚轴和子叶在含有 2 ,4 D(添加有BA或激动素 )的培养基上可诱导三种典型不同的愈伤类型 .其中 ,来源于下胚轴的颗粒状的、松脆的胚性愈伤在分化培养基上可获得高频率的再生小植株 .讨论了不同类型的愈伤组织和其对应的分化再生率之间的关系 .此外 ,本研究探讨了来源于粗皮桉的下胚轴、子叶及成熟叶片在添加有一系列BA和NAA的不同浓度组合的B5培养基上的器官发生途径 ,三种不同的外植体在不同的再生培养基上的最高再生频率不同 ,其中成熟叶片、子叶和下胚轴的最高再生率逐渐提高 ,分别为 4 1 6 7%、6 6 6 7%和 83 33% .该文还讨论了两种不同再生系统的意义

Plant Regeneration in Eucalyptus pellita

In this study, two different approaches of regeneration systems for explants in Eucalyptus pellita have been taken to establish an efficient differentiation and regeneration protocols. The somatic embryogenesis from in vitro hypocotyls and cotyledons in Eucalyptus pellita has been investigated. The typical three kinds of callus types were induced from hypocotyls and cotyledons cultured on the 2,4-D containing medium with BA or kinetin.High frequency (82 % ) plantlet production from the friable, granular embryogenic callus derived from hypocotyls has been obtained on the differentiation medium( 1μM TDZ; 1μM BA plus 0. ltMNAA ) developed in this study.The relationship between the callus types formed on different callus induction media and their corresponding differ entiation rate was further discussed. In addition, plant regeneration through organogenesis from in vitro hypocot yls, cotyledons and mature leaves in Eucalyptus pellita has also been conducted with the modified basal B5 medi um supplemented with a range of combinations of BA and NAA. The highest frequency of shoot formations for three kinds of explants varied on the different regeneration medium, with the regeneration rate for leaves, cotyle dons and hypocotyls in ascending order: 41.67%, 66.67% and 83.33%, respectively. The significance of the two different plant regeneration systems was discussed. To our knowledge, this is the first report on the regenera tion of Eucalyptus pellita.