| 适合RT-PCR的苹果芽RNA快速提取方法研究 |
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| 引用本文： | 张立莎,彭建营. 适合RT-PCR的苹果芽RNA快速提取方法研究[J]. 安徽农业科学, 2009, 37(25): 11885-11885 |
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| 作者姓名： | 张立莎 彭建营 |
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| 作者单位： | 河北农业大学园艺学院,河北保定,071001;河北农业大学园艺学院,河北保定,071001 |
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| 基金项目： | 河北省自然科学基金项目 |
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| 摘 要： | [目的]从苹果单芽中高效、快速提取RNA，为后续分子生物学研究奠定基础。[方法]分别以苹果品种新红星、嘎拉和富士的单芽为材料，用改良SDS法提取RNA，并经过甲醛变性琼脂糖凝胶电泳和RT—PCR检测RNA质量。[结果]研究出一种高效、快速的苹果单芽RNA提取方法，该方法能在2．5h内得到完整的RNA，经RT—PCR检验，能获得理想的目的片段。[结论]该方法稳定可靠，适合苹果单芽RNA提取。
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| 关 键 词： | 苹果芽 RNA提取 RT-PCR |
Study on a Rapid Extraction Method of RNA from Apple Bud for RT-PCR |
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| ZHANG Li-sha et al. Study on a Rapid Extraction Method of RNA from Apple Bud for RT-PCR[J]. Journal of Anhui Agricultural Sciences, 2009, 37(25): 11885-11885 |
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| Authors: | ZHANG Li-sha et al |
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| Affiliation: | ZHANG Li-sha et al (College of Horticulture,Agricultural University of Hebei,Baoding,Hebei 071001) |
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| Abstract: | [Objective] The study aimed to establish an effective and rapid protocol for RNA extraction from single apple bud,which was benificial to subsequent molecular biological research.[Method] RNA from apple cultivars 'Starkrimson','Gala' and 'Fuji' buds was extracted by modified-SDS method,and RNA was detected by formaldehyde denaturing agarose gel electrophoresis and RT-PCR.[Result] An effective and rapid RNA extraction protocol was established,which could be completed in 2.5 h.RNA extracted through this proto... |
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| Keywords: | RT-PCR |
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