Determination of lignin,holocellulose, and organic solvent extractives in fresh leaf,litterfall, and organic material on forest floor using near-infrared reflectance spectroscopy

We report the results of a study regarding the near-infrared reflectance spectra of various leaf stages from fresh to senescing, and to decomposing leaf. A broad absorbance feature increased in the region of 1100–1400nm with the advance of the leaf senescence and leaf decomposition. A decrease was seen in the region over 1440nm during the senescence and decomposition process. These differences of spectra showed the changes in constituents of leaf in terms of the degree of the senescence and decomposition. A comparison of multiple linear regression between the near-infrared reflectance spectra and proximate chemical analyses showed that near-infrared reflectance spectroscopy achieved a certain level of useful accuracy. We consider that near-infrared reflectance spectroscopy has the potential to predict the contents of carbon fractions in plant materials, and that this method can replace previous methods due to faster determination of carbon fractions, and its ability to significantly increase the number of samples that can be collected and measured.