| 兰州百合花瓣高频再生体系研究 |
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| 引用本文： | 刘芬.兰州百合花瓣高频再生体系研究[J].北方园艺,2007(6):210-212. |
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| 作者姓名： | 刘芬 |
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| 作者单位： | 甘肃省农科院果树研究所,兰州,730070 |
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| 摘 要： | 以兰州百合为试材,研究总结出了利用花瓣建立外植体的高频再生体系:用花瓣建立外植体时,灭菌时间控制在15min效果较佳,污染率、失活率均低;适宜诱导花瓣愈伤组织的培养基为MS BA0.5mg/L 2,4-D 0.5mg/L,诱导率达到90%;适宜芽分化培养基为MS BA1.5mg/L NAA0.1mg/L,分化率为75%;适宜继代和增殖的培养基为MS BA1.5mg/L IBA0.5mg/L,增殖倍数可达5.8;生根培养基以GS NAA0.25mg/L最佳,生根率高达100%,试管苗生长势强,且根系发达;用草炭作移栽基质,成活率高,为89.6%.
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| 关 键 词： | 兰州百合 花瓣 诱导 继代 生根 基质 |
| 文章编号： | 1001-0009（2007）06-0210-03 |
| 收稿时间： | 2007-03-28 |
| 修稿时间： | 2007-03-28 |
Research on Micropropagation from Petals of Lanzhou Lily |
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| LIU Fen.Research on Micropropagation from Petals of Lanzhou Lily[J].Northern Horticulture,2007(6):210-212. |
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| Authors: | LIU Fen |
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| Institution: | Pomology Institute of Gansu Academy of Agricultural Scienee,Lanzhou 730070 |
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| Abstract: | The technique of micropropagation from petals of Lanzhou Lily was built.The results showed that 15 minutes explants sterilized time had better effect on lower contamination rate,MS BA0.5mg/L 2,4-D 0.5mg/L was suitable for petal callus induction,MS BA1.5mg/L NAA 0.1mg/L was suitable for adventitious shoot formation,the optimum medium for subculture was MS BA1.5mg/L IBA0.5mg/L,and the best medium for root regeneration was GS NAA0.25mg/L with 100% root generation rate and vigorous roots.The living rate of transplanted seedling on pasteurized peat was 89.6%. |
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| Keywords: | Lanzhou Lily Petal induction Subculture Root regeneration Medium |
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