条叶百合和垂花百合高效快繁体系的建立

以条叶百合和垂花百合的鳞片为外植体,应用均匀设计法对各步骤各主要因子和水平的作用进行了试验探讨.结果表明:条叶百合鳞茎诱导最适宜的培养基为N6+0.05 mg.L-1IAA+0.01 mg.L-1NAA+0.50 mg.L-1KT,诱导率为95.5%,鳞茎萌发为完整植株的最佳培养基为N6+0.01 mg.L-1IAA,萌发率为94%;垂花百合鳞茎诱导最适宜的培养基为N6+0.05 mg.L-1IAA+0.50 mg.L-1KT,诱导率为95%,鳞茎萌发为完整植株的最佳培养基为N6+0.01 mg.L-1IAA+0.001 mg.L-1NAA,萌发率为92.5%.以鳞茎的切片为材料进行快繁的结果表明,在35-40 d的一个培养周期内,增殖系数达10以上.对不同阶段培养材料的形态及超微结构的观察证明了2种百合的鳞茎发生发育过程.

Efficient micropropagation system of Lilium callosum and Lilium cernum

The scales of Lilium callosum Sieb.et Zucc.and Lilium cernum Kom.were used as explants.The effects of main factors of various levels at each step were investigated through uniform design experiments.The results showed that N6+0.05 mg·L-1 IAA+0.01 mg·L-1 NAA+0.50 mg·L-1 KT was most suitable for L.callosum with an induction rate of 95.5%,N6+0.01 mg·L-1 IAA was most suitable for L.callosum with a germination rate of 94%;N6+0.05 mg·L-1 IAA+0.50 KT mg·L-1 was most suitable for L.cernum with an induction rate of 95%,N6+0.010 mg·L-1 IAA+0.001 mg·L-1 NAA was most suitable for L.cernum with a germination rate of 92.5%.Slices each with one node were cut from induced bulblets and cultured for propagation,and a 10-fold proliferation rate was achieved within 35-40 days.The observation of morphological structure and ultrastructure proved the process of inducing bulblets and bulblets germination of the two kinds of lily.