Utility of the discriminant analysis to categorize untrained Spanish Pure Bred horses on the basis of biochemical and muscle variables before and after a standardized exercise test |
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| Institution: | 1. Norwegian University of Life Sciences, Faculty of Veterinary Medicine and Biosciences, Department of Production Animal Clinical Sciences, Kyrkjevegen 332-334, 4325 Sandnes, Norway;2. Norwegian Veterinary Institute, Department of Laboratory Services, Postbox 750 Sentrum, NO-0106 Oslo, Norway;3. Norwegian Veterinary Institute, Department of Health Surveillance, Postbox 750 Sentrum, NO-0106 Oslo, Norway;1. Department of Biological and Pharmaceutical Veterinary Sciences, Comparative Anatomy Unit/URAC49, Hassan II Agronomy and Veterinary Institute, Rabat, Morocco;2. Jumenterie de Zouada, Veterinary Hospital- Royal Guard, Tetouan, Morocco;3. Veterinary Hospital- Royal Guard, Rabat-Morocco, Morocco;4. Department of Medicine, Surgery and Reproduction, PMC- EC, Hassan II Agronomy and Veterinary Institute, Rabat, Morocco;1. Department of Veterinary Pharmacology and Toxicology, Faculty of Veterinary Medicine, Ataturk University, Erzurum, Turkey;2. Department of Veterinary Pathology, Faculty of Veterinary Medicine, Ataturk University, Erzurum, Turkey;3. Department of Medical and Molecular Genetics, King''s College London, London, United Kingdom;4. Laboratory of Forensic Medicine and Toxicology, School of Medicine, Aristotle University of Thessaloniki, Greece;5. Department of Analytical and Forensic Medical Toxicology, Sechenov University, 2-4 Bolshaya Pirogovskaya st., 119991 Moscow, Russia;6. Department of Pathology–Cytopathology, Medical School, University of Crete, Heraklion, Greece;7. Department of Toxicology & Forensic Sciences, Faculty Medicine, University of Crete, Heraklion, Greece |
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| Abstract: | We hypothesized that the information obtained from a discriminant analysis could be used to objectively discriminate horses untrained from early ages, in agreement with certain physiological characteristics.In the biopsies of 24 Spanish Pure Bred horses (1.5–3 years old) before and after a standardized exercise test (SET; 4–7 m/s with a change of velocity of 1 m/s every 2 min) muscle enzymes, substrate and metabolites were determined. Also, diverse plasma and blood parameters were considered.Three pre-exercise groups (A1: six horses; A2: seven horses and A3: eleven horses) and two post-exercise groups (B1: sixteen horses; B2: eight horses) were defined from a correspondence analysis. Forward stepwise discriminant analysis selected 11 variables which differentiated the groups between each other both pre- and post-exercise.The results of the present study suggested the utility of a discriminant analysis to categorize horses in agreement with certain physiological variables. It could be used for establishing different types of training in each group by expert trainers. |
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