Abundance and community structure of ammonia-oxidizing bacteria and archaea in a temperate forest ecosystem under ten-years elevated CO2 |
| |
| Institution: | 1. State Key Laboratory of Urban and Regional Ecology, Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, 18 Shuangqing Road, Beijing 100085, China;2. Environmental Futures Centre and Griffith School of Environment, Griffith University, Nathan, Queensland 4111, Australia;3. Environmental Futures Centre and School of Biomolecular and Physical Sciences, Griffith University, Nathan, Queensland 4111, Australia;4. Nicholas School of the Environment and Earth Science, Duke University, Durham, NC 27708, USA;5. Department of Forest Ecology and Management, Swedish University of Agricultural Sciences (SLU), SE-901 83 Umeå, Sweden;1. Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographical Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China;2. National Engineering Research Center for Information Technology in Agriculture, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China;3. University of Chinese Academy of Sciences, Beijing 101408, China;4. Harrow Research and Development Center, Agriculture & Agri-Food Canada, Harrow, Ontario N0R 1G0, Canada;1. Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150081, China;2. University of Chinese Academy of Sciences, Beijing 100049, China;3. Institute of Soil and Fertilizer and Environment Resources, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China;4. Institute of Agricultural Resource and Environment, Jilin Academy of Agricultural Sciences, Changchun 130033, China;5. Heihe Branch of Heilongjiang Academy of Agricultural Sciences, Heihe 164300, China;1. Institute of Resource, Ecosystem and Environment of Agriculture, Nanjing Agricultural University, Nanjing 210095, P.R.China;2. Guilin University of Technology, Guilin 541004, P.R.China;1. Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, No. 1799 Jimei Road, Xiamen 361021, China;2. Ningbo Key Lab of Urban Environment Processes and Pollution Control, Ningbo Urban Environment Observation and Research Station—NUEORS, Chinese Academy of Sciences, No. 88 Zhongke Road, Ningbo 315830, China;3. Nanjing Institute of Agricultural Sciences in Jiangsu Hilly Area, No. 6 Xianyin South Road, Qixia District, Nanjing, Jiangsu 210046, China;4. Research Center for Environmental Ecology and Engineering, Wuhan Institute of Technology, Wuhan 430073, China |
| |
| Abstract: | Ammonia-oxidizing bacteria (AOB) and archaea (AOA) are considered as the key drivers of global nitrogen (N) biogeochemical cycling. Responses of the associated microorganisms to global changes remain unclear. This study was to determine if there was a shift in soil AOB and AOA abundances and community structures under free-air carbon dioxide (CO2) enrichment (FACE) and N fertilization in Duke Forest of North Carolina, by using DNA-based molecular techniques, i.e., quantitative PCR, restriction fragment length polymorphism (RFLP) and clone library. The N fertilization alone increased the abundance of bacterial amoA gene, but this effect was not observed under elevated CO2 condition. There was no significant effect of the N fertilization on the thaumarchaeal amoA gene abundance in the ambient CO2 treatments, while such effect increased significantly under elevated CO2. A total of 690 positive clones for AOA and 607 for AOB were selected for RFLP analysis. Analysis of molecular variance (AMOVA) indicated that effects of CO2 enrichment and N fertilization on the community structure of AOA and AOB were not significant. Canonical correspondence analysis also showed that soil pH rather than elevated CO2 or N fertilization shaped the distribution of AOB and AOA genotypes. A negative linear relationship between the δ13C and archaeal amoA gene abundance indicated a positive effect of elevated CO2 on the growth ammonia oxidizing archaea. On the other hand, the community structures of AOB and AOA are determined by the soil niche properties rather than elevated CO2 and N fertilization. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
