Water-extractable soil organic matter inhibits phosphatase activity |
| |
| Affiliation: | 1. Henan Key Laboratory of Polyoxometalate Chemistry, Institute of Molecular and Crystal Engineering, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan 475004, PR China;2. State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, PR China |
| |
| Abstract: | We show that water-extractable soil organic matter may inhibit activity of added phosphatase. The inhibition was greater for the topsoils than the corresponding subsoils. The enzyme kinetics in buffer and in soil solutions followed Michaelis–Menten kinetics. Vmax decreased and Km increased with increasing soil water-extractable organic carbon. Both kinetic parameters followed similar trends with the intensities of both fluorescence peaks of the solutions. The effect was more marked at pH below the optimum pH. Inhibition was complex, particularly for topsoil solutions where Vmax was decreased and Km increased. This effect could lead to underestimation of catalytic activity of enzymes extracted from soil and could result in the overestimation of decreased specific activity in the adsorbed phase of enzymes added to soils. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
