Measurements of microbial community activities in individual soil macroaggregates |
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| Affiliation: | 1. Environmental Microbiology Unit, Department of Public Health, University of Patras, 26500 Patras, Greece;2. Environmental Engineering Laboratory, Department of Civil Engineering, University of Patras, 26500 Patras, Greece;3. School of Environmental Engineering, Technical University of Crete, 73100 Chania, Greece;1. W.K. Kellogg Biological Station, Michigan State University, Hickory Corners, MI 49060, USA;2. UMR Eco&Sols, Campus Supagro, 2 place Viala, Montpellier, 34060 France;3. Department of Plant Biology, Department of Integrative Biology, and Program in Ecology, Evolutionary Biology and Behavior, Michigan State University, East Lansing, MI 48824, USA;1. Key Laboratory of Wetland Ecology and Environment & Jilin Provincial Joint Key Laboratory of Changbai Mountain Wetland and Ecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China;2. Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China;3. School of Engineering, Edith Cowan University, Joondalup, WA 6027, Australia;4. University of Chinese Academy of Sciences, Beijing 100049, China;1. W. K. Kellogg Biological Station, Michigan State University, 3700 East Gull Lake Drive, Hickory Corners, MI 49060, USA;2. Population Biology Graduate Group, University of California, Davis, 2320 Storer Hall, One Shields Avenue, Davis, CA 95616, USA;3. Department of Plant Biology, Michigan State University, 612 Wilson Road, East Lansing, MI 48824-1312, USA;4. Program in Ecology, Evolutionary Biology, and Behavior, Michigan State University, 293 Farm Lane, East Lansing, MI 48824-1312, USA |
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| Abstract: | The functional potential of single soil macroaggregates may provide insights into the localized distribution of microbial activities better than traditional assays conducted on bulk quantities of soil. Thus, we scaled down enzyme assays for β-glucosidase, N-acetyl-β-d-glucosaminidase, lipase, and leucine aminopeptidase to measure of the enzyme potential of individual macroaggregates (250–1000 μm diameter). Across all enzymes, the smallest macroaggregates had the greatest activity and the range of enzyme activities observed in all macroaggregates supports the hypothesis that functional potential in soil may be distributed in a patchy fashion. Paired analyses of ATP as a surrogate for active microbial biomass and β-glucosidase on the same macroaggregates suggest the presence of both extracellular β-glucosidase functioning in macroaggregates with no detectable ATP and also of relatively active microbial communities (high ATP) that have low β-glucosidase potentials. Studying function at a scale more consistent with microbial habitat presents greater opportunity to link microbial community structure to microbial community function. |
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