Prevalence and genetic characterization of Giardia and Cryptosporidium in cats from Italy |
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| Institution: | 1. Division of Infectious Diseases, Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, IL, USA;2. Department of Pathology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA;1. Department of Microbiology and Parasitology, Biomedical Institute, Fluminense Federal University, Rua Professor Hernani Pires de Melo 101, Centro, Niterói, RJ 24210-130, Brazil;2. Department of Statistics, Institute of Mathematics and Statistics, Fluminense Federal University, Rua Professor Marcos Waldemar de Freitas Reis s/n, Blocos G e H, Campus do Gragoatá, São Domingos, Niterói, RJ 24210-201, Brazil |
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| Abstract: | One hundred and eighty one cats living in central Italy were tested for the presence of Giardia and Cryptosporidium infection by IFAT test and specific PCRs. Overall eight (4.4%) samples were IFAT-positive for Giardia. All the IFAT-positive samples for Giardia scored positive for the PCRs, and three more samples IFAT-negative generated PCR products leading to a total 6.1% molecular positivity rate for Giardia. All the examined samples were negative for Cryptosporidium. Sequencing of samples molecularly positive to Giardia indicated that three cats harbored the zoonotic Giardia duodenalis Assemblage A, whereas all other positive animals were infected with the feline-specific G. duodenalis Assemblage F. Phylogenetic analysis carried out on the sequences obtained supported the clustering of the isolates within Assemblages A and F. The results here presented provide data on the occurrence of Giardia genotypes in cats living in close contact with humans highlighting the potential importance of this protozoan disease for the public health. |
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