Assessment of the arbuscular mycorrhizal fungal community in roots and rhizosphere soils of Bt corn and their non-Bt isolines |
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| Institution: | 1. Key Laboratory of Ecological Agriculture, Ministry of Agriculture, South China Agricultural University, Guangzhou 510642, China;2. Key Laboratory of Agroecology and Rural Environment of Guangdong Regular Higher Education Institutions, South China Agricultural University, Guangzhou 510642, China;3. Department of Ecology, College of Agriculture, South China Agricultural University, Guangzhou 510642, China;1. Faculty of Sciences, J. E. Purkinje University, České mládeže 8, Ústí nad Labem, CZ-40096, Czech Republic;2. Institute of Microbiology, v.v.i., Academy of Sciences of the Czech Republic, Vídeňská 1083, Prague 4, CZ-14220, Czech Republic;3. Institute of Bio- and Geosciences, IBG-3: Agrosphere, Forschungszentrum Jülich GmbH, D-52425 Jülich, Germany;1. Health Physics and Dosimetry Research Laboratory, Department of Energy Engineering and Physics, Amirkabir University of Technology, Tehran, Iran;2. Department of Medical Physics, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran;1. Department of Chemical Biology, Fakultät für Biologie, Centre for Medical Biotechnology, University Duisburg-Essen, Universitätsstraße 2, 45117 Essen, Germany;2. Department of Bioengineering Sciences, Microbiology Unit, Vrije Universiteit Brussel and VIB Department of Structural Biology, Pleinlaan 2, 1050 Brussels, Belgium;3. Plant Chemetics Lab, Max-Planck-Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, 50829 Cologne, Germany;1. College of Science, National University of Defense Technology, Changsha 410073, Hunan, China;2. Beijing Institute of Pharmaceutical Chemistry, Beijing 102205, China;1. Friedrich Schiller University Jena, Institute of Geosciences, Applied Geology, Burgweg 11, 07749 Jena, Germany;2. Friedrich Schiller University Jena, Institute of Inorganic and Analytical Chemistry, Environmental Analysis, Lessingstraße 8, 07743 Jena, Germany;3. Helmholtz Centre for Environmental Research – UFZ, Soil Physics, Lysimeter Station Falkenberg, Dorfstraße 55, 39615 Falkenberg, Germany;4. Helmholtz Centre for Environmental Research – UFZ, Water Analytics & Chemometrics, Brückstraße 3a, 39114 Magdeburg, Germany |
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| Abstract: | Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) in combination with sequencing of amplified partial 18S ribosomal DNA was used to assess the effects of transgenic Bt corn 5422Bt1 (event Bt 11) and 5422CBCL (event MON810) on the community structure of a non-target microorganism, namely the arbuscular mycorrhizal fungi (AMF) Glomus in corn roots and rhizosphere soils, relative to their non-Bt isolines 5422 (conventional parent) and 5422wx (conventional hybrid). AMF colonization in roots of different corn genotypes was also assessed using microscopic visualization. No adverse effect was detected on the indigenous AMF colonization of the roots of Bt hybrids 5422Bt1 and 5422CBCL. Two-way indicator species analysis (TWINSPAN®) and detrended correspondence analysis (DCA) of the DGGE data from corn roots presented differences between Bt and non-Bt corn isolines (5422Bt1 vs. 5422wx, and 5422CBCL vs. 5422wx). However, differences were also recognized between the two non-Bt corn cultivars (5422 vs. 5422wx), and between the two Bt corn lines (5422Bt1 vs. 5422CBCL) in roots. Our results suggest that corn genotypes may have a greater influence on the AMF community structure of plant roots and rhizosphere soils than other factors, such as the age of the growing plants. |
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