Mineral fertilizer alters cellulolytic community structure and suppresses soil cellobiohydrolase activity in a long-term fertilization experiment |
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| Affiliation: | 1. Key Laboratory of Plant Nutrition and Fertilization, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, South Zhongguancun Street No. 12, Beijing 100081, China;2. AgResearch Ltd, Lincoln Science Centre, Christchurch 8140, New Zealand;3. Laboratory of Nutrients Recycling, Institute of Applied Ecology, Chinese Academy of Sciences, 72 Wenhua Road, Shenyang 110016, China;1. Institute of Soil Science and Site Ecology, Dresden University of Technology, Pienner Str. 19, 01737 Tharandt, Germany;2. Institute of Physicochemical and Biological Problems in Soil Science, Russian Academy of Sciences, Institutskaya 2, 142290 Pushchino, Russia;3. Department of Soil Science of Temperate Ecosystems, University of Göttingen, Büsgenweg 2, 37077 Göttingen, Germany;4. Institute of Landscape Ecology, WWU – University of Münster, Heisenbergstraße 2, 48149 Münster, Germany;1. College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, PR China;2. Institute of Soil and Water Conservation, Northwest A&F University, Yangling 712100, PR China;3. College of Geoscience and Survey Engineering, China University of Mining and Technology (Beijing), Beijing 100083, China;1. State Key Laboratory of Forest and Soil Ecology, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China;2. Soil Science of Temperate Ecosystems, Büsgen-Institute, Georg August University of Göttingen, Büsgenweg 2, 37077 Göttingen, Germany;3. Key Laboratory of Regional Environment and Eco-remediation, College of Environment, Shenyang University, Shenyang 110044, China;4. Department of Earth, Atmospheric, and Planetary Sciences, Purdue University, West Lafayette, IN 47907, USA;5. Department of Agronomy, Purdue University, West Lafayette, IN 47907, USA;6. University of Chinese Academy of Sciences, Beijing 100049, China;1. Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture / Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China;2. Nafferton Ecological Farming Group, Newcastle University, Nafferton Farm, Stocksfield, Northumberland NE43 7XD, UK;1. Jiangsu Provincial Coordinated Research Center for Organic Solid Waste Utilization, Nanjing Agricultural University, Nanjing, 210095, China;2. Department of Biology, University of York, Wentworth Way, York, YO10 5DD, UK;3. Crop Research Institute, Anhui Academy of Agricultural Science, Hefei, 230031, China |
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| Abstract: | Nutrient inputs to soil can alter mineralization of organic matter and subsequently affect soil carbon levels. To understand how elemental interactions affect the biogeochemistry and storage of soil C, we examined soils receiving long-term applications of mineral fertilizer and manure-containing fertilizers. As cellulose is the dominant form of carbon entering arable soils, cellulolytic communities were monitored through enzymatic analysis, and characterization of the abundance (real-time PCR) and diversity (terminal restriction fragment length polymorphism, T-RFLP) of fungal cellobiohydrolases (cbhI) genes. The data showed that long-term mineral fertilization increased soil organic C and crop productivity, and reduced soil heterotrophic respiration and cellobiohydrolases (CBH) activity. Correspondingly, the diversity and community structure of cellulolytic fungi were substantially altered. The variation in cellulolytic fungi is mainly attributable to shifts in the proportion of Eurotiomycetes. In addition, CBH activity was significantly correlated with the diversity and community structure of cellulolytic fungi. These results suggest that enhanced C storage by mineral fertilizer addition occurs not only from extra organic carbon input, but may also be affected through the cellulose decomposing community in arable soil. |
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