Isolation of polymorphic microsatellite markers in Aporrectodea icterica (Savigny 1826) |
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Affiliation: | 1. U.S.G.S.—Great Lakes Science Center, Tunison Laboratory of Aquatic Science, 3075 Gracie Road, Cortland, NY 13045, USA;2. New York State Department of Environmental Conservation, 317 Washington Street, Watertown, NY 13601, USA;3. Ontario Ministry of Natural Resources, Glenora Fisheries Station, R.R 4, Picton, Ontario, KOK2TO, Canada |
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Abstract: | Earthworms play a major role in soil dynamics acting as modifiers of properties and soil characteristics. Although population genetics is a promising approach to get a better understanding of their ecology, the use of molecular tools in earthworm studies is still scarce. Here, we developed and analyzed seven microsatellite loci for Aporrectodea icterica, a common endogeic species of most temperate natural and agricultural soils, with the aim to investigate its dispersal capacity in further researches. Although sequences of a fragment of the mitochondrial gene cytochrome oxidase subunit I (COI) showed the existence of two mitochondrial lineages within the species, with a mean divergence between them of 10%, microsatellite data proved that these two COI lineages are interbreeding and form part of a single species. |
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