| 奶牛布鲁氏菌病微量凝集试验诊断方法的建立 |
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| 引用本文： | 王双山,王真,马延光,冯现明,吴清民.奶牛布鲁氏菌病微量凝集试验诊断方法的建立[J].中国畜牧兽医,2013,0(5):206-210. |
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| 作者姓名： | 王双山 王真 马延光 冯现明 吴清民 |
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| 作者单位： | 1. 安阳工学院, 河南安阳 455000;2. 中国农业大学动物医学院, 北京 100193;3. 河南渑池县动物疫病控制中心, 河南渑池 472400;4. 林州市动物疫病控制中心, 河南林州 456550 |
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| 基金项目： | 农业部行业科技项目(200903027)。 |
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| 摘 要： | 为探索操作简便、快速、实用的奶牛布病诊断方法,本研究建立了奶牛布鲁氏菌病(布病)诊断96孔V型板微量凝集方法,并优化了试验条件。结果显示,抗原的最佳稀释浓度为1:20,反应最适温度为37℃,反应时间为24 h;为验证本研究建立的微量凝集方法的准确性,对120份奶牛布病虎红平板凝集试验阳性血清样品进行微量法凝集和试管凝集试验,结果显示,微量法与虎红平板凝集试验的符合率为83.3%,试管法与虎红平板凝集试验符合率为80.0%。提示,微量凝集试验可以作为试管凝集试验的替代方法,应用于规模化奶牛场布病的诊断和检疫。
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| 关 键 词： | 布鲁氏菌病 奶牛 微量凝集试验 诊断 |
| 收稿时间： | 2012-10-26 |
Establishment of Microscale Agglutination Test for Diagnosis of Cow Brucellosis |
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| WANG Shuang-shan,WANG Zhen,MA Yan-guang,FENG Xian-ming,WU Qing-min.Establishment of Microscale Agglutination Test for Diagnosis of Cow Brucellosis[J].China Animal Husbandry & Veterinary Medicine,2013,0(5):206-210. |
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| Authors: | WANG Shuang-shan WANG Zhen MA Yan-guang FENG Xian-ming WU Qing-min |
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| Institution: | 1. Anyang Institute of Technology, Anyang 455000, China;2. College of Veterinary Medicine, China Agricultural University, Beijing 100193, China;3. Mianchi Animal Disease Control Center, Mianchi 472400, China;4. Linzhou Animal Disease Control Center, Linzhou 456550, China |
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| Abstract: | To explore simple, fast and practical diagnosis method for cow brucellosis in this study, we established a microscale agglutination test using 96-well "V" plate and optimized the test conditions, the optimal antigen diluted concentration was 1:20, the optimal reaction temperature was 37℃, and the optimal reaction time was 24 h. To verify the veracity microscale agglutination test, we retested 120 rose-bengal plate agglutination test (RBT) positive cow serum samples by microscale agglutination test and tube agglutination test, the coincidence rate of microscale agglutination test and tube agglutination test to RBT were 83.3% and 80.0%, respectively. These results showed that the microscale agglutination test could be used as the alternative method of tube agglutination test for cow brucellosis diagnosis and quarantine. |
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| Keywords: | brucellosis cow microscale agglutination test diagnosis |
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