卵泡抑素基因过表达对其通路上相关基因表达的影响

为了探讨过表达卵泡抑素(follistatin,FST)基因对其通路上相关基因表达量的影响,本研究采用PCR方法扩增了民猪的FST基因,构建真核表达载体pcDNA3.1(+)-Dsig-FST,对转染了重组质粒的成纤维细胞进行了鉴定,并采用实时荧光定量PCR检测通路上相关基因表达量的变化。结果表明,本研究成功克隆了民猪的FST基因,并在猪胎儿成纤维细胞中显著过表达,结果导致了ActRⅡB和BMP4基因显著下调 (P<0.05),Smad4和Myostatin基因有下调趋势,ActRⅡA基因有上调趋势。由此可见,FST基因的表达变化对其通路上部分相关基因的表达有不同程度的抑制或促进作用。

Effect of FST Gene Over-expression on Expression of Related Genes in its Pathway

To investigate the effect of follistatin (FST) gene over-expression on related genes in its pathway, this study cloned the FST gene of Min pig with PCR method, constrcuted eukaryotic expression vector pcDNA3.1(+)-Dsig-FST, identified fibroblast cells transfected with the recombinant plasmid, and detected the change of related genes in its pathway with Real-time PCR method. The results showed that FST gene of Min pig was successfully cloned and over-expressed significantly in the porcine fetal fibroblast cell, and then lead to ActRⅡB and BMP4 gene decline significantly (P<0.05),Smad4 and Myostatin gene had downward trend, ActRⅡA gene had the upward trend. In summary, FST gene changed in its expression had the different effect of degrees inhibition or promotion on its related genes.