贵州省某规模化猪场暴发猪瘟与链球菌病混合感染的诊断

为了解贵州某规模化猪场发病断奶仔猪死亡原因,本研究采用流行病学调查、临床症状观察、病理解剖诊断和RT-PCR检测等方法,对该规模化猪场发病猪进行了诊断。试验结果表明,通过流行病学调查、剖检病理和猪瘟抗体快速金标检测卡检测,初步诊断该猪场发病猪疑似猪瘟病毒和细菌混合感染;RT-PCR检测核酸确诊发病猪为猪瘟病毒感染;细菌培养分离、生化特性鉴定和动物致病性试验确诊为猪链球菌。造成该猪场断奶仔猪发病死亡的原因为猪瘟病毒和猪链球菌混合感染所致。

Diagnosis of Mixed Infection of Classic Swine Fever (CSF) and Streptococcicosis on an Intensive Pig Farm in Guizhou Province

In order to determine the reason of weaned piglets’ death on an intensive pig farm in Guizhou province, the infected weaned piglets from this pig farm were detected using epidemiological survey, autopsy pathological observation, anatomic pathological diagnosis and RT-PCR method. The results showed that from epidemiological survey and autopsy pathological observation, we primarily diagnosed the pig farm, which was suspected infected by classic swine fever virus (CSFV) and bacteria. The RT-PCR result confirmed that the infected pigs were infected by CSFV and the bacterium was confirmed as Streptococcus suis by bacteria culture and isolation, biochemical character identification and animal pathogenicity test. Comprehensive above test results showed that mixed infection of CSFV and Streptococcus suis were the cause of the pig death in this pig farm.