牛源溶血性巴氏杆菌的分离鉴定

乌鲁木齐某肉牛养殖场育肥牛发生了以体温升高、呼吸困难、咳嗽及快速死亡为主要特征的疾病,每日死亡率超过5%。从发病死亡牛肝脏和肺脏等组织中分离到1株细菌,通过菌落形态、染色特性、培养特性及生化试验等一系列鉴定,确定为溶血性巴氏杆菌。给小白鼠腹腔注射该菌悬液,8 h内全部死亡,结合该批次牛刚从伊犁长途运输而来的流行病学特点,诊断该病是以溶血性巴氏杆菌为主要病原的牛运输热。药敏试验结果表明沙拉沙星和强力霉素最敏感,将它们用于全群预防和治疗后效果显著。

Isolation and Identification of Bovine Origin Pasteurella hemolytica

A diseases occurred in a beef cattle fattening farm in Urumqi area,characterised by higher body temperature,dyspnea,coughing and could cause rapid death,daily mortality more than 5%. One bacterial strain was isolated from liver and lung samples of new dead cattle,the bacterial was identified as Pasteurella hemolytica according to cultivation characters,colonial morphology,stain peculiarity and biochemistry test.5 experimental rats received intraperitoneal injection of the bacterial suspension,the result was that all of them died within 8 hours.Combine the epidemiological fact that these cattles just came to Urumqi after long-distance transportation from Yili,so the diagnosis of the disease was bovine shipping fever caused by Pasteurella hemolytica.Drug sensitivity test showed that sarafloxacin hydrochloride and doxycycline were most sensitive, we used these kinds of drugs to the whole herd,it showed good treatment and prevention effect.