吴茱萸组织培养和快速繁育技术研究 |
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引用本文: | 唐征,荆赞革,张小玲,罗天宽,刘庆,朱世扬. 吴茱萸组织培养和快速繁育技术研究[J]. 安徽农业科学, 2012, 0(36): 17475-17477 |
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作者姓名: | 唐征 荆赞革 张小玲 罗天宽 刘庆 朱世扬 |
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作者单位: | 浙南作物育种重点实验室,温州科技职业学院生物工程技术研究所,浙江温州 325006 |
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摘 要: | [目的]对吴茱萸的组织培养和快速繁育技术进行研究.[方法]以吴茱萸的雌株嫩叶为外植体,研究不同的植物生长调节剂和浓度配比对其诱导、增殖和生根培养的影响.[结果]最佳诱导培养基为MS+ 1.0 mg/L BA +0.3 mg/L NAA,诱导率可达74.4%,且幼苗正常,无玻璃化现象;最佳增殖培养基为MS+2.0 mg/L BA +0.1 mg/L KT +0.3 mg/L NAA,增殖系数可达4.61;以1/2MS+ 2.0 mg/LNAA为生根培养基,生根率可达86%,平均生根数为5.4.[结论]该研究建立了吴茱萸的快速繁殖体系,为其优良雌性系的工厂化苗木生产提供了技术支撑.
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关 键 词: | 吴茱萸 组织培养 快速繁殖 |
Study on Tissue Culture and Rapid Propagation of Tetradium ruticarpum |
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Affiliation: | TANG Zheng et al(Southern Zhejiang Key Laboratory of Crop Breeding,Biotechnology Research Institution,Wenzhou Vocational College of Science and Technology,Wenzhou,Zhejiang 400031) |
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Abstract: | [Objective] The paper was to study the tissue culture and rapid propagation of Tetradium ruticarpum.[Method] Using the tender leaf from female plant of Tetradium ruticarpum as explants,the effects of different plant growth regulator and concentration on the induction,proliferation,rooting culture were studied.[Result] The appropriate adventitious bud inducing medium of Tetradium ruticarpum was MS+1.0 mg/L BA+0.3 mg/L NAA,the inductivity was 74.4%,and the seedling was normal and had no vitrification phenomenon.The best medium for proliferation was MS+2.0 mg/L BA+0.1 mg/L KT+0.3 mg/L NAA,the propagation coefficient was 4.61.The rooting rate could reach to 86% by using 1/2MS+2.0 mg/L NAA as rooting medium,the average rooting number was 5.4.[Conclusion] This study established the Tetradium ruticarpum rapid propagation system,and provided technical support for the industrial seedling production of its excellent female line. |
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Keywords: | Tetradium ruticarpum Tissue culture Rapid Propagation |
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