厚竹叶绿素荧光成像的异质性研究

目的]为了研究厚竹叶片叶绿素荧光的异质性.方法]运用调制荧光成像系统Mini-IMAGING-PAM的叶绿素荧光和成像技术,测量厚竹叶片叶绿素荧光,计算各荧光参数.结果] PSⅡ最大量子产量(Fυ/Fm)和叶片吸光系数(Abs)均质性较高,实际量子产量(Y)、非光化学淬灭(NPQ/4)、光化学淬灭(qP)和相对电子传递速率(PS/50)的异质性都很强.Y、qP和PS/50的变化趋势基本相同,横向呈现两边高、中间低的趋势,即靠近叶片边缘数值高,靠近叶脉部分数值低.叶片纵向(从上到下)的Y、qP和PS/50变异系数逐渐减小,说明从叶子尖端到叶子基部异质性逐渐降低.PS/50和NPQ/4呈相反变化趋势.结论]厚竹叶片各荧光参数有不同的异质性.

Study on Heterogeneity of Chlorophyll Fluorescence Imaging of Phyllostachys edulis cv. Pachyloen

Objective] This study aimed to explore the heterogeneity of chlorophyll fluorescence of Phyllostachys edulis cv.Pachyloen leaves.Method] Using the chlorophyll fluorescence system and fluorescence imaging system in the Mini-IMAGING-PAM,the chlorophyll fluorescence of Phyllostachys edulis cv.Pachyloen leaves were measured and the fluorescence parameters were calculated.Result] The homogeneity of the maximal quantum yield of PS II(Fv/Fm) and the leaf absorptivity(Abs) were higher,with CVs(Coefficient of Variation) of 1.58%-1.68% and 1.75%-2.12% respectively,while the heterogeneity of other fluorescence parameters,including the actual quantum yield(Y),non-photochemical quenching(NPQ/4),photochemical quenching(qP) and relative photosynthetic rate(PS/50) were higher,with CVs of 9.60%-14.23%,10.23%-13.02%,11.92%-13.02% and 11.15%-17.74% respectively.The trends of heterogeneity change in Y,qP and PS/50 were basically the same with laterally higher heterogeneity at two edges and lower heterogeneity in the middle,namely with larger CV at the margin and smaller one around the midrib.Longitudinally,the variation coefficients of Y,qP and PS/50 decreased gradually(from top to bottom),which indicated that the heterogeneity declined from the leaf tip to the base.The trends of heterogeneity change in PS/50 and NPQ/4 were opposite.Conclusion] The fluorescence parameters of Phyllostachys edulis cv.Pachyloen leaves revealed different heterogeneity.