| PRV gE抗原表位基因的序列分析 |
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| 引用本文： | 石迎新,罗满林,黄毓茂,刘镇明. PRV gE抗原表位基因的序列分析[J]. 华南农业大学学报, 2002, 23(4): 71-74 |
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| 作者姓名： | 石迎新 罗满林 黄毓茂 刘镇明 |
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| 作者单位： | 1. 深圳出入境检验检疫局动检处
2. 华南农业大学兽医学院,广东,广州,510642 |
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| 基金项目： | 广东省科技攻关资助项目 (ZKM0 35 0 7N)，国家自然科学基金资助项目 (39770 0 33) |
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| 摘 要： | 设计，合成了1对特异笥引物，以PRV－MinA和PRV－YueA株的病毒基因组为模板扩增gE的抗原表位基因，均获得了约560bp的特异性产物，将PCR产物克隆到pUCm-T载体上，构建重组质粒pUCMAgE和pUCYAgE。经菌落PCR和质粒酶切鉴定后，将目的基因进行序列测定。结果表明：目的基因均为558bp组成，编码186个氨基酸；核酸序列和推导的氨基酸序列的同源性分析表明，PRV－YueA和PRV－MinA株具有相对较低的同一性，分别为96．9％和92．5％。
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| 关 键 词： | PRV gE抗原表位基因 序列分析 伪狂犬病毒 家畜 野生动物 疾病防治 |
| 文章编号： | 1001-411X(2002)04-0071-04 |
| 修稿时间： | 2001-12-07 |
Sequence Analysis of gE Antigen Epitope Genes of Pseudorabies Virus |
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| SHI Ying xin,LUO Man lin,HUANG Yu mao,LIU Zhen ming. Sequence Analysis of gE Antigen Epitope Genes of Pseudorabies Virus[J]. JOURNAL OF SOUTH CHINA AGRICULTURAL UNIVERSITY, 2002, 23(4): 71-74 |
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| Authors: | SHI Ying xin LUO Man lin HUANG Yu mao LIU Zhen ming |
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| Abstract: | A pair of primers were designed and synthesized. Using the whole genomes of strain PVR MinA and PRV YueA as templates,specific products were obtained as expected by PCR amplification and cloned into pUCm T vector.Recombinants were confirmed by colony PCR and restriction enzyme digestion.The inserts were sequenced,and the results revealed that each of both inserts composed of 558 neucleotides,coding for 186 amino acids.Comparison of the target genes showed 96 9% nuleotide sequence homology and 92.5% amino acid sequence homology between strain PRV YueA and PRV MinA,which is relatively low. |
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| Keywords: | pseudorabies virus gE antigen epitope gene sequence analysis |
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