加工番茄子叶和下胚轴离体植株再生的研究

以加工番茄BO 2和BO 4无菌苗的子叶和下胚轴为外植体,以M S为基本培养基,研究了不同浓度的ZT,6-BA与不同浓度的IAA组合对外植体芽诱导的影响。结果表明,在不同浓度的ZT和IAA组合中,对2个番茄品种的子叶和下胚轴外植体芽诱导均以M S+1.0 m g/L ZT+0.05 m g/L IAA培养基为最好,BO 2品种子叶和下胚轴的出芽率分别为32.7%和24.5%,BO 4品种的分别为37.3%和28.2%;在不同浓度的6-BA和IAA组合中,诱导BO 2外植体出芽率最高的培养基是M S+2.0 m g/L 6-BA+0.2 m g/L IAA,子叶和下胚轴的出芽率分别为27.3%和14.5%;诱导BO 4品种子叶和下胚轴出芽率最高的培养基分别为M S+1.0 m g/L 6-BA+0.2 m g/LIAA和M S+2.0 m g/L 6-BA+0.2 m g/L IAA,子叶和下胚轴的出芽率均为28.2%;在M S+0.1 m g/L IAA培养基上,加工番茄BO 2再生芽1周左右就能长出根,形成完整的小植株。

Study on cotyledon and hypocotyl plant regeneration of processing tomato in vitro

Cotyledons and hypocotyls of BO2 and BO4 process tomatoes were used as explants to study the effects of different concentrations of ZT,6-BA combined with different concentrations of IAA on bud induction.The results showed that the best shoot induction medium for cotyledon and hypocotyls explants was MS+ZT 1.0 mg/L+IAA 0.05 mg/L in different combinations of ZT and IAA.The shoot induction ratios were 32.7% and 24.5% for BO2 cotyledon and hypocotyls explants and 37.3% and 28.2% for BO4 cotyledon and hypocotyls explants respectively.Among different combinations of 6-BA and IAA,the best medium for BO2 was MS+6-BA 2.0 mg/L+IAA 0.2 mg/L and the shoot induction ratio was 27.3% and 14.5% for cotyledon and hypocotyls explants respectively.The most suitable induction medium for cotyledon and hypocotyls explants of BO4 was MS+6-BA 1.0 mg/L+IAA 0.2 mg/L and MS+6-BA 2.0 mg/L+IAA 0.2 mg/L respectively and the shoot induction ratio was 28.2%.The root could be induced after the regenerated buds were planted in MS+IAA 0.1 mg/L one week later.