黄皮种子中杀松材线虫成分分离及活性测定

采用药液浸渍法测定了黄皮Clausena lansium不同部位甲醇提取物对松材线虫Bursaphelenchus xylophilus的生物活性,在活性跟踪的基础上,通过萃取、柱层析、薄层制备、重结晶、核磁共振等方法对其有效成分进行了分离和结构鉴定.结果表明,黄皮不同部位甲醇提取物中种子提取物对松材线虫的毒杀活性最高,在1 mg/mL水溶液中处理72 h的校正死亡率为100%.各萃取相中石油醚相的活性最高,从石油醚相中分离、纯化获得一黄色晶体,经核磁共振检测分析为Lansiumamide B(N-甲基-N-顺-苯乙烯-肉桂酰胺).该化合物处理松材线虫24、48、72 h的LC50值分别为8.38、6.36、5.38 mg/L,表明该化合物是黄皮种子中主要杀松材线虫活性成分.

Active Ingredients of Seed Extracts of Clausena lansium and Nematocidal Activity Against Bursaphelenchus xylophilus

Nematocidal activity of methanol extracts from the different parts of Clausena lansium was determined by dipping method.The active ingredients were isolated and identified on the basis of active-directed fractionation with column chromatography,thin layer chromatography(TLC),recrystallization and nuclear magnetic resonance(NMR).The results showed that the most effective extract against Bursaphelenchus xylosphilus was seed methanol extract,which gave 100% mortality after 72 h treatment at the mass concentration of 1 mg/mL.The active ingredients were concentrated in petroleum ether fraction and a yellow crystal was obtained and purified.It was identified as Lansiumamide B(N-methyl-N-cis-styryl-cinnamamide) on the basis of NMR data.The bioassay results revealed that LC50 values of Lansiumamide B against B.xylophilus were 8.38,6.36 and 5.38 mg/L after 24,48 and 72 h treatment,respectively.The results indicated that Lansiumamide B was effective against B.xylophilus.