基于HPLC法的向日葵盘中绿原酸含量测定

［目的］采用HPLC法测定向日葵盘绿原酸的含量。［方法］采用高效液相色谱法。其中，色谱柱为Hypersil ODS（4.6 mm×200 mm，5 μm），甲醇-冰醋酸-乙腈（18∶35∶2）为流动相，流速为1.0 ml/min，检测波长为327 nm。［结果］绿原酸在3.24～89.21 μg/ml范围内线性关系良好,回归方程为：C=23.63A-3.98,r=0．999 8，平均回收率为98.12％，RSD=0.27％。［结论］所建方法准确可靠，操作简便，稳定性好，可用于绿原酸生产的质量控制。

Determination of Ahlorogenic Acid in Sunflower Plate Based on High Performance Liquid Chromatography

Objective]The research aimed to determine chlorogenic acid content in sunflower plate based on using HPLC method.Method] Chlomgenic acid was determined by HPLC method.Hypersil ODS(4.6 mm×200 mm,5 μm)was used and the mobile phase consisted of methanol-glacial acetic acid-acetonitrile(18∶35∶2))at a flow rate of 1.0 ml/min.The detection wavelength was set on 327 nm.Result] The linear range of chlorogenic acid was 3.24-89.21 μg/ml.The regression equation was C=23.63A-3.98,(r=0.999 8).The average recovery was 98.12%,with RSD of 0.27%.Conclusion] The method is simple,accurate and stable,and it can be used for the quality control of sunflower plate.