阔叶猕猴桃叶片离体器官发生和植株再生(英文)

以阔叶猕猴桃(A ctinidia latifolia M err.)叶片为外植体,通过器官发生途径诱导形成不定芽,探讨了不同激素组合、暗培养时间以及不同浓度的蔗糖对叶片器官发生和植株再生的影响。结果表明,BW +0.1μm ol/L N AA +5μm ol/L Zeatin 附加20g/L 蔗糖,先进行21d 暗培养然后转到光下培养效果最好,6周后不定芽再生频率达91.67%,平均每个外植体再生芽数6.87个。再生芽生根良好,生根率可达100%。首次报道了阔叶猕猴桃的器官发生和植株再生,建立了叶片的高效再生体系,为今后的遗传转化研究奠定了基础。

In vitro organogenesis and plant regeneration from leaf explants of Actinidia latifolia

An efficient plant regeneration protocol for A ctinidia latifolia was achieved via organogenesis from leaf explants in vitro. In this paper, effects of different combinations of growth regulators, dark treatments and sucrose concentrations on the organogenesis from leaf explants were examined. After 6 weeks of culture maximum shoot regeneration frequency of 91.67%with 6.87 shoots per explant was obtained on BW medium containing 0.1 μmol/L α-naphthaleneacetic acid, 5 μmol/L zeatin and 20 g/L sucrose incubated in the dark for 21 days then exposured to light. When cultured in darkness no explant regenerated shoots. However, shoots appeared quickly in the following week after exposure to light in the dark treatments for 14 d, 21 d and 28 d. It is considered that light is essential for shoot regeneration from leaf explants of A. latifolia. The lower sucrose concentration (10 g/L) promoted the formation of small and stunted shoots. Higher sucrose concentrations (≥30 g/L)had a detrimental effect on shoot regeneration, and 20 g/L sucrose gave the best results. The highest rooting rate reached 100% together with the maximum roots per explant (17.53) on BW medium containing 0.1 μmoL/L 6-benzyladenine and 10μmol/L indolebutyric acid. Plantlets with good root system were successfully acclimatized to the field conditions and produced healthy plants. To our knowledge, this is the first report of an in vitro regeneration method for A. latifolia. The results of this study will facilitate the application of genetic transformation methods in A. latifolia.