氟乐灵诱导甜瓜四倍体研究初探

氟乐灵是一种新型的具有染色体加倍功效的除草剂。该试验通过氟乐灵溶液处理刚刚发芽的甜瓜种子诱导四倍体,在室温条件下对甜瓜品种黄河蜜三号,以200,400,600倍浓度的氟乐灵溶液处理甜瓜种子4,6,8 h进行四倍体诱导试验,发现氟乐灵处理的根尖有明显的加粗现象。通过镜检,染色体计数法测定诱导率1],结果表明,浓度为400倍的氟乐灵溶液处理露白的甜瓜种子8 h所获得的加倍效果最好,诱导率达到32.2%。试验结果表明,氟乐灵处理甜瓜种子可以成为获得四倍体甜瓜的有效途径之一,同时对其他作物的多倍体育种研究提供了参考依据。

The Preliminary Explore on Induction of Tetraploidin Muskmelon with Trifluralin

Triflfralin is a Patten herbicide.It is reported that Trifluralin have function of making double chromosomes.In the experiment muskmelon seeds of 3# yellow river honeydew in immersing with Thifluralin at 200 times,400 times,and 600 times for 4 h,6 h,8 h respectively were treated under room temperature.The roots tip that treated with Trifluralin were increased obviously in diameter by observed directly.The cell root tip were inspected by microscope.The result showed that the best treatment for inducing tetraploid were obtained by immersing seeds showing valuables unintentionally with trifluralin solution at 400 times,when the muskmelons seeds were immersed for 8 h,the induction frequency being 32.2%.The method that seeds were immersed with trifluralin solution was a new and efficient way to obtain tetraploid muskmelons.Meanwhile,the achievements of experiment provide a new guiding basis for ployploid breeding research work of other crops.