Comparative analysis of organelle DNAs in acid citrus grown in Japan using PCR-RFLP method |
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Authors: | Asad Asadi Abkenar Shiro Isshiki Ryoji Matsumoto Yosuke Tashiro |
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Institution: | (1) Laboratory of Plant Breeding and Biotechnology, Citrus Research Institute, P O Box 46915-335, Ramsar, Iran;(2) Faculty of Agriculture, Saga University, Saga 840-8502, Japan |
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Abstract: | PCR-RFLP analyses of three regions for each of chloroplast DNA (cpDNA; rbcL-ORF106, trnD-trnT, trnH-trnK) and mitochondrial DNA (mtDNA; nad7/exon2-exon3, nad7/exon3-exon4, 18S-5S) were performed in 26 cultivars of acid citrus grown in Japan to identify polymorphisms and classify them. The polymorphisms
were compared with those of three true Citrus species, i.e., mandarin, pummelo and citron. Ichang papeda (C. ichangensis) was also included in this study to find its relationship with Yuzu. Inter-species cpDNA variation was recognized and the
acid citrus were divided into three groups, namely; I (‘Yuzukichi’ and ‘Kinkoyu’), II sour oranges (‘Kaiseito’, ‘Daidai’
and ‘China daidai’), ‘Nansho daidai’, ‘Kiku daidai’, C. sudachi (‘Mushi yukaku’, ‘Yushi yukaku’ and ‘Yushi mukaku’), C. sphaerocarpa (‘Kabosu’ and ‘Aka kabosu’), C. kizu (‘Taninaka kizu’, ‘Kinosu’ and ‘Kizu’), ‘Zanbo’, ‘Mochiyu’, ‘Jabara’ and ‘Naoshichi’], and III Yuzu (‘Tetraploid’, ‘Tochikei
yuzu’ and ‘Yamanekei yuzu’), ‘Matsuda sudachi’, ‘Zuishoyu’, ‘Hanayu’ and ‘Yuko’]. CpDNA restriction patterns of the three
true Citrus species differed from each other as well as from those of ichang papeda. CpDNA restriction patterns of group I of the acid
citrus were identical to those of mandarins. Group II showed the same as pummelos. CpDNA restriction patterns of group III
were differed from those of the three true Citrus species in the three regions. This group was differed from ichang papeda after digestion of trnH-trnK PCR products with TaqI, HinfI and AluI, while they showed identical restriction patterns in two regions, rbcL-ORF106 and trnD-trnT. Citrons and ichang papeda were placed in groups IV and V, respectively. Based on mtDNA restriction patterns, the acid citrus
were divided into three groups; i, ii and iii. In groups i and ii accessions of groups I and II of cpDNA were placed with
mandarins and pummelos, respectively. In group iii accessions of group III of cpDNA were placed with ichang papeda. Citrons
were placed in a distinct group, iv. |
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Keywords: | Acid citrus Chloroplast DNA Mitochondrial DNA PCR-RFLP |
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