番茄不同分化潜力愈伤组织中内源多胺比较及外源多胺对分化的影响

比较了番茄具芽分化活性愈伤组织（ＢＤＣ）、根分化活性愈伤组织（ＲＤＣ）和不具分化活性愈伤组织（ＮＤＣ）中内源多胺含量的变化．结果表明：在培养的第６天和１２天，多胺总量均是ＢＤＣ＞ＲＤＣ＞ＮＤＣ．ＢＤＣ在肉眼可见芽分化的前３天（培养第１２天），其精胺、腐胺、尸胺含量显著升高，精胺含量分别为ＲＤＣ和ＮＤＣ的２．３倍和４．１倍；腐胺含量分别为ＲＤＣ和ＮＤＣ的２．４倍和１．５倍；尸胺含量分别为ＲＤＣ和ＮＤＣ的１．８倍和１．３倍．结合外源多胺的使用结果表明：外源尸胺和腐胺抑制芽的分化但促进根的分化，精胺对芽的分化有促进作用但对根的分化无效．提出了激素对离体培养物形态建成的调节作用可能通过改变内源多胺含量及其比例起作用．

Comparison of endogenous polyamines contents among distinct calli and influence of exogenous polyamines on organogenesis of tomato explants in vitro

This paper was intended to compare endogenous polyamines contents among distinct calli of tomato ( Lycopersicon esculentum Mill) explants in vitro, i e, bud differentiation callus (BDC), root differentiation callus (RDC), non differentiation callus(NDC) In addition, influence of exogenous polyamines on organogenesis was also investigated The results showed that on the 6th and 12th day in culture, total polyamines in content was BDC > RDC> NDC Just before visible bud appeared in BDC (12th day after inoculation), spermin (spm), cadverine (cad) and putrescine(put) in quantity increased significantly in comparison with RDC or NDC Spm content in BDC on the 12th day inoculated was 2 3 and 4 1 times, while cad was 1 8 and 1 3 times, put, 2 4 and 10 5 times, than that in RDC and NDC, respectively Promotion of bud differentiation was observed by addition of foreign spm However, addition of exogenous put or cad gave rise to root formation It was supposed that hormonal regulation of organogenesis in culture was intermediated through changing polyamines contents and ratios among them