| 球根海棠"金正日"的离体培养研究 |
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| 引用本文： | 唐中彦,刘淼.球根海棠"金正日"的离体培养研究[J].安徽农业科学,2007,35(10):2906-2906,3035. |
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| 作者姓名： | 唐中彦 刘淼 |
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| 作者单位： | 辽东学院实验中心,辽宁丹东,118003 |
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| 摘 要： | 通过诱导胚状体建立组织培养再生系统,研究了球根海棠"金正日"的离体培养.结果表明,外植体经HgCl2溶液表面消毒后,接种到MS+6-BA 0.2 mg/L+NAA 1 mg/L培养基上诱导愈伤组织,15 d后转接到1/2 MS+6-BA 0.5 mg/L +NAA 0.1 mg/L培养基上,诱导胚状体,诱导率90%以上.胚状体在MS+6-BA 0.3 mg/L +NAA 0.03 mg/L培养基上以丛生芽形式增殖,增殖系数达4～8倍.试管苗生根可采用MS+IBA 0.1 mg/L、蔗糖2%培养基.生根率100%.
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| 关 键 词： | 球根海棠 金正日 胚状体 组织培养 |
| 文章编号： | 0517-6611（2007）10-02906-01 |
| 收稿时间： | 2007-01-09 |
| 修稿时间： | 2007-01-09 |
Studies on in Vitro Culture of Begonia Tuberhybrida Jinzhengri |
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| TANG Zhong-yan, et al.Studies on in Vitro Culture of Begonia Tuberhybrida Jinzhengri[J].Journal of Anhui Agricultural Sciences,2007,35(10):2906-2906,3035. |
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| Authors: | TANG Zhong-yan |
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| Institution: | Centre of Laboratory, Eastern Liaoning University, Dandong, Liaoning 118003 |
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| Abstract: | The tissue culture and regenerate system of Jinzhengri were established through inducting embryo.The explants with surface sterilization of HgCl2 were inoculated on the MS medium containing 6-BA 0.2 mg/L NAA 1 mg/L for inducting callus.After 15 days,the calli were removed on the medium with 1/2 MS 6-BA 0.5 mg/L NAA 0.1 mg/L for inducting the embryo,with the induction rate of above 90%.The embryos were propagated in form of cespitose buds on the medium with MS 6-BA 0.3 mg/L NAA 0.03 mg/L,with the propagation coefficient reaching 4 to 8 times.The root induction rate of test-tube seedlings was 100% on the medium with MS IBA 0.1 mg/L 2% saccharose. |
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| Keywords: | Begonia tuberhybrida Jinzhengri Embryo Tissue culture |
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