| 禽源多杀性巴氏杆菌多位点序列分型研究 |
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| 引用本文： | 李伟杰. 禽源多杀性巴氏杆菌多位点序列分型研究[J]. 中国兽药杂志, 2017, 51(2): 1-6 |
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| 作者姓名： | 李伟杰 |
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| 作者单位： | 中国兽医药品监察所 |
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| 基金项目： | 生物安全关键技术研发（2016YFC1201603）；国家微生物资源平台（NIMR-5） |
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| 摘 要： | 为了解国内禽源多杀性巴氏杆菌流行情况,对分离自18省份的84株多杀性巴氏杆菌采用荚膜多重PCR分型和多位点序列分型对其血清型和基因型进行鉴定。结果表明:禽源多杀性巴氏杆菌主要以血清A型为主,占96.4%(81/84);多位点序列分型可将禽源多杀性巴氏杆菌分为5种ST型,其中ST129为主要流行型,占94.0%(79/84)。本研究为我国禽源多杀性巴氏杆菌的流行病学监测和基因多样性提供了数据支持。
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| 关 键 词： | 多杀性巴氏杆菌；荚膜分型；多位点序列分型 |
| 收稿时间： | 2016-11-21 |
| 修稿时间： | 2017-02-16 |
Multi-locus Sequence Typing of Pasteurella multocida Isolated from Avian |
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| Affiliation: | China Institute of Veterinary Drug Control |
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| Abstract: | To investigate the epidemic status of the Pasteurella multocida isolated from avian, eighty-four P. multocida isolates collected from 18 provinces were subject to identifications of the serotypes and genotypes by capsular multiplex PCR and multi-locus sequence typing. The results showed that P. multocida isolated from avian were mainly classified into serotype A, accounting for 94.6%. Five different sequence types including ST129, ST122, ST107, ST62, ST44 were identified by MLST, and the predominate genotype was ST129, accounting for 94.6%. The present study provides data support for the epidemiological surveillance and genetic diversity of P. multocida isolated from avian. |
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| Keywords: | Pasteurella multocida capsular serotyping multi-locus sequence typing |
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