| 黄瓜再生体系和遗传转化的建立 |
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| 引用本文： | 叶永亮,杜波. 黄瓜再生体系和遗传转化的建立[J]. 黑龙江农业科学, 2009, 0(5): 5-7 |
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| 作者姓名： | 叶永亮 杜波 |
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| 作者单位： | 哈尔滨市农业科学院,黑龙江哈尔滨,150070 |
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| 摘 要： | 以黄瓜（Cucumis sativus L.）哈研3号为试验材料,采用组织培养技术系统探讨以子叶或子叶节为外植体时不定芽的诱导效率,最终确定以子叶节作为再生体系及遗传转化体系的试验材料;并系统探讨了不同PGRs浓度对不定芽诱导、不定芽伸长及生根阶段的影响;建立了哈研3号黄瓜的高频再生体系;并在子叶节根端膨大阶段进行了抗生素（Km）浓度的摸索,建立了较为适宜的遗传转化体系,为转基因工作打下坚实的基础。
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| 关 键 词： | 黄瓜 子叶节 不定芽 再生体系 遗传转化体系 PGRs |
Construction of Cucumber Regeneration System and Genetic Transformation System |
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| YE Yong-liang,DU Bo. Construction of Cucumber Regeneration System and Genetic Transformation System[J]. Heilongjiang Agricultural Science, 2009, 0(5): 5-7 |
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| Authors: | YE Yong-liang DU Bo |
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| Affiliation: | Harbin Academy of Agricultural Sciences;Harbin;Heilongjiang 150070 |
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| Abstract: | Taking cucumber(Cucumis sativus L.) "Hayan No.3" as the test material,using tissue culture technology system to explore induction efficiency of adventitious shoot for cotyledon or cotyledon node as explants.Then,the cotyledon node as a regeneration system and genetic transformation system was determined as test materials.And systems of different PGRs concentration on adventitious bud induction,adventitious shoot elongation and rooting stages of impact were discussed.Finally,the"Hayan No.3" the high-frequenc... |
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| Keywords: | PGRs |
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