| 蜜蜂囊状幼虫病RT-PCR快速检测方法的初步应用 |
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| 引用本文： | 周亮,宋战昀,王全凯,刘金华,薛立刚,王振国. 蜜蜂囊状幼虫病RT-PCR快速检测方法的初步应用[J]. 蜜蜂杂志, 2010, 30(6): 9-10 |
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| 作者姓名： | 周亮 宋战昀 王全凯 刘金华 薛立刚 王振国 |
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| 作者单位： | 1. 吉林农业大学,吉林,长春,130118
2. 吉林出入境检验检疫局,吉林长春,130062 |
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| 摘 要： | 蜜蜂的囊状幼虫病是中华蜜蜂常见且为害严重的疾病,病原为小正链RNA病毒。实验通过RT-PCR的方法检测出了该病毒,经序列测定发现与广东的蜜蜂囊状幼虫病毒基因序列有所差别,推测可能是由于地区和蜜蜂种类差异导致的。
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| 关 键 词： | 蜜蜂囊状幼虫病 RT—PCR |
RT-PCR Detection for the Honey bee Sacbrood Virus Disease |
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| ZHOU Liang,SONG Zhan-yun,WANG Quan-kai,LIU Jin-hua,XUE Li-gang,WANG Zhen-guo. RT-PCR Detection for the Honey bee Sacbrood Virus Disease[J]. Journal of Bee, 2010, 30(6): 9-10 |
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| Authors: | ZHOU Liang SONG Zhan-yun WANG Quan-kai LIU Jin-hua XUE Li-gang WANG Zhen-guo |
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| Affiliation: | 1. JiLin Agricultural University, Changchun,130118,China; 2 JiLin Entry-Exit Inspect ion and Quarantine Bureau, Changchun,130062,China) |
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| Abstract: | Honey bee sacbrood virus （SBV） is a kind of picornavirus , a small positive strand RNA virus , which is greatly threatened to the Chinese honeybee breeding. This experiment detect the virus with the method of RT-PCR, and found the difference between the sacbrood virus sequence of UK and GUANDONG province with sequence determination, suspect that because of the different area and the kind of honeybee. |
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| Keywords: | RT-PCR |
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