西芹根物质浸提液处理后黄瓜叶片内几种氮代谢物质含量的变化

以黄瓜品种"津春4号"为材料,在黄瓜植株第1片真叶长至宽5 cm时,使用西芹鲜根及根际区物的乙醇、丙酮和蒸馏水浸提液对黄瓜植株进行化感处理(灌根),测定处理后黄瓜叶片内几种氮代谢物质含量的变化,从氮代谢角度研究西芹鲜根及根际区物浸提液处理后黄瓜植株对黄瓜枯萎病菌的化感抑制作用机理。结果表明:处理后黄瓜叶片内可溶性蛋白质、氨、丝氨酸、精氨酸、脯氨酸与总氨基酸含量均高于对照,含量达到峰值时分别较丙酮对照增加10.58%、18.31%、6.54%、3.10%、9.64%和3.16%,而硝酸盐、甘氨酸和蛋氨酸含量则低于对照,含量达到最低值时分别较丙酮对照降低7.52%、3.41%和15.55%。对于不同西芹根物质浸提液处理,丙酮浸提液处理的氮代谢物质含量变化最显著,乙醇浸提液处理次之,蒸馏水浸提液处理变化最低,西芹鲜根浸提液处理的氮代谢物质含量变化高于西芹根际区物浸提液处理。

Nitrogen metabolites in cucumber leaves treated with parsley root extracts

Previous studies have noted that parsley extracts had allelopathic effect on Fusarium oxysporium f. sp. Cucumerinum with an inhibitory rate of 12.89%~84.83%. It has also been noted that different fresh parsley root and root soil extracts had different prohibitive allelopathic strengths against cucumber wilt. With steep decline in morbidity, however, the prohibitive allelopathic strengths were within 5.45%~46.95%. Concerns have been reported on the mechanism of parsley extract allelopathy, including protective enzyme systems, soluble substances and active oxygen metabolism of cucumber leaf. However, no concerns have been reported in relation to nitrogen metabolites in cucumber leaf. To study the mechanisms of fresh parsley root and root soil extract allelopathy on F. oxysporium f. sp. Cucumerinum, changes in nitrogen metabolites were analyzed in cucumber seedlings treated with extracts once its first leaf was 5 cm wide. In contrast to earlier studies, this study showed that nitrogen metabolites in "Jinchun 4" cucumber leaves treated with fresh parsley root and root soil extracts were significantly changed. The contents of soluble protein, ammonia, Ser, Arg, Pro and amino acids were increased compared to the control, with the highest increases over the acetone control by 10.58%, 18.31%, 6.54%, 3.10%, 9.64% and 3.16%, respectively. However, nitrate, Gly and Met contents dropped, with the greatest declines of 7.52%, 3.41% and 15.55%, respectively. For the different treatments, the most significant effects on nitrogen metabolites were noted by acetone extracts, followed by ethanol extracts and distilled water extracts. Fresh root extracts caused more distinct changes in nitrogen metabolite contents than root soil extracts.