| 鲢InDel-RFLP标记开发及其在连锁图谱构建中的应用 |
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| 引用本文： | 张鹏,杨官品,张立楠,危起伟,邹桂伟.鲢InDel-RFLP标记开发及其在连锁图谱构建中的应用[J].淡水渔业,2011,41(2):43-50,29. |
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| 作者姓名： | 张鹏 杨官品 张立楠 危起伟 邹桂伟 |
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| 作者单位： | 中国海洋大学海洋生物遗传育种教育部重点实验室;中国水产科学研究院长江水产研究所; |
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| 基金项目： | 农业部淡水鱼类种质资源与生物技术重点开放实验室开放课题(LFB20070601); 现代农产品加工技术研发体系专项基金(nycytx-49-01) |
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| 摘 要： | 基于随机测序的鲢(Hypophthbalmichthys molitrix)基因组片段,尝试开发基于InDel的限制性片段长度多态性标记(InDel-RFLP).结果显示:共133个片段中,65个在一个野生鲢群体(该野生鲢群体采自长江流域荆州段,31个个体)中表现多态性.共检测到163个等位基因,每位点等位基因数从2到...
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| 关 键 词： | 鲢(Hypophthalmichthys molitrix) 碱基插入缺失 限制性片段长度多态性 连锁图谱 |
InDel-RFLPs of silver carp (Hypophthalmichthys molitrix) and their application to constructing the linkage map |
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| ZHANG Peng,YANG Guan-pin,ZHANG Li-nan,WEI Qi-wei,ZOU Gui-wei.InDel-RFLPs of silver carp (Hypophthalmichthys molitrix) and their application to constructing the linkage map[J].Freshwater Fisheries,2011,41(2):43-50,29. |
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| Authors: | ZHANG Peng YANG Guan-pin ZHANG Li-nan WEI Qi-wei ZOU Gui-wei |
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| Institution: | ZHANG Peng1,YANG Guan-pin1,ZHANG Li-nan1,WEI Qi-wei2,ZOU Gui-wei2 (1.Key Laboratory of Marine Genetics and Breeding of Chinese Ministry of Education,Ocean University of China,Qingdao,Shandong 266003,2.Yangtze River Fisheries Research Institute,Chinese Academy of Fishery,Wuhan 430223) |
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| Abstract: | In order to detour the limitations faced in the development of SSR markers,133 randomly sequenced genomic fragments were used to develop InDel based restriction fragment length polymorphism(InDel-RFLP) markers for silver carp(Hypophthalmichthys molitrix).65 fragments were polymorphic in a wild silver carp population containing 31 individuals collected from Jingzhou fragment(the middle reach) of Yangtze River.In total,163 alleles were identified.The allele number per locus varied from 2 to 4 with an average ... |
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| Keywords: | silver carp(Hypophthalmichthys molitrix) insertion-deletion restriction fragment length polymorphism linkage map |
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