Structural insights to investigate Conypododiol as a dual cholinesterase inhibitor from Asparagus adscendens |
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Authors: | Inamullah Khan Muhammad Nisar Nematullah Khan Muhammad Saeed Said Nadeem Fazal-ur-Rehman Farooq Ali Nasiara Karim Waqar Ahmad Kaleem Mughal Qayum Hanif Ahmad Ihsan Ali Khan |
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Affiliation: | a Department of Pharmacy, University of Peshawar, Peshawar 25120, Pakistan;b Institute of Chemical Sciences, University of Peshawar, Peshawar 25120, Pakistan;c H.E.J. Research Institute of Chemistry, International Center for Chemical Sciences, University of Karachi, Karachi, Pakistan;d Faculty of Pharmacy, Gomal University D.I. Khan, Pakistan;e Wensom College, Gomal University D.I. Khan, Pakistan |
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Abstract: | The main aim of the current study was to explore molecular insights for potentially new dual cholinesterase inhibitor(s) from Asparagus adscendens via molecular docking. This medicinal plant is traditionally used as a nerve tonic and remedy for memory impairments. Conypododiol was isolated from the chloroform fraction of methanolic extract of A. adscendens, based on bioactivity guided isolation. Conypododiol exhibited significant inhibition of both acetylcholinesterase and butyrylcholinesterase, having the IC50 values 2.17 ± 0.1 μM and 11.21 ± 0.1 μM, respectively. IC50 values of the standard compound Galanthamine for both the enzymes were 0.537 ± 0.018 μM and 8.6 ± 0.27 μM, respectively. Based on MTT cytotoxicity assay, Conypododiol was found safe against LCMK-2 monkey kidney epithelial cells and mice hepatocytes. Molecular docking studies revealed the hydrogen bonding interactions of Conypododiol with His440 and Ser200 at esteratic site (ES), and also with Tyr334 at peripheral anionic site (PAS) of the aromatic gorge of acetylcholinesterase. Simultaneous contacts of Conypododiol with PAS and ES shows its significance as a bivalent ligand. This preliminary study highlighted the potential of Conypododiol to be further developed and modified as new lead compound identified by its folk use. |
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Keywords: | Molecular docking Acetylcholineterase Butyrylcholinesterase Conypododiol Asparagus adscendens |
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