新疆棉花子叶节直接诱导多芽发生再生植株的研究

以中国三大棉花产区之一的新疆棉花中长绒陆地棉和特色长绒棉品种为试验材料,采用子叶节作外植体,为防止陆地棉品种外植体褐化的影响,以分步添加6-BA来启动和/或诱导多芽的发生,此时用葡萄糖作为碳源代替通常使用的蔗糖,经过伸长培养后,以1/2 MS培养基添加10 mg/L BA诱导生根,生根率可达72.2;.并通过对不同品种诱导多芽发生的比较,认为以子叶节为外植体直接诱导多芽发生受棉花品种的基因型影响不大,为用农杆菌介导法规模化遗传改良棉花奠定基础.

Study on Regenerative Plant of Direct Multiple Shoots from Colyledon Nodes in Xinjiang Cotton

The paper deals with the Gossypium hirsutum L.varieties and Gossypium barbadense L.varieties of Xinjiang that is one of three largest cotton production region in China.Cotyledon Nodes act as the explants in vitro 6-BA of plant cytokinins is added to start-up medium and/or induce medium by two steps for preventing Gossyqpum hirsutum L.from browning effect on explants.Meanwhile,glucose serves as carbon resource in place of sucrose used usually.After elongating culture of shoots,rooting was induced in one half of MS medium added 10 mg/L BA and its ratio can reach 72.2%.Through comparing induction of multiple shoots of different varieties,experiment infers that in vitro induction of direct multiple shoots and plant regeneration from cotyledon nodes had less affect on variety genotype, which laid the foundation of improveing cotton by agrobacterium-mediated and large-scale transformation.