| 甘蓝类蔬菜亲缘关系的RAPD初步分析 |
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| 引用本文： | 田源,王超.甘蓝类蔬菜亲缘关系的RAPD初步分析[J].中国蔬菜,2008,1(1):20-22. |
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| 作者姓名： | 田源 王超 |
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| 作者单位： | 东北农业大学园艺学院 150030 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 利用RAPD标记从DNA水平上对30份甘蓝类蔬菜材料进行亲缘关系和遗传多样性分析，从100对引物中选出10对扩增效果较好的引物，用其扩增出90条带，其中多态条带72条，多态率为80.0 %。对RAPD结果进行UPGMA分析，结果表明，在相似系数0.85处可以清楚的将它们划分为6组，并且从分子生物学的角度分析了它们之间的亲缘关系。
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| 关 键 词： | 甘蓝类蔬菜 RAPD 遗传多样性 亲缘关系 |
| 收稿时间： | 2007-06-05 |
| 修稿时间： | 2007-10-20 |
Initial Analysis on Genetic Relationship of Brassica oleracea L. By RAPD |
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| Tian Yuan,Wang Chao.Initial Analysis on Genetic Relationship of Brassica oleracea L. By RAPD[J].China Vegetables,2008,1(1):20-22. |
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| Authors: | Tian Yuan Wang Chao |
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| Institution: | (College of Horticulture,Northeast Agricultural University，Harbin 150030) |
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| Abstract: | RAPD analysis on genetic relationship and genetic diversity of 30 samples of Brassica oleracea L.was conducted.Ten primers were selected from 100 primers to amplify 30 samples,and a total number of 90 bands wereamplified,among which 72(80.0 %)were polymorphic.The results of RAPD were analyzed by UPGMA clustering.The result showed that all samples could be divided into 6 groups with the similarity coefficient of 0.85,and their genetic relationship was analyzed. |
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| Keywords: | Brassica oleracea L RAPD Genetic diversity Genetic relationship |
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