添加不同发情时期山羊血清对牛胚胎体外发育的影响

为了提高牛体外胚胎囊胚发育率,添加不同发情时期山羊血清对牛胚胎进行体外培养。分别采集发情周期D0、D2、D4和D7山羊血清（发情当天为D0）,灭活除菌备用。结果表明不同发情时期羊血清对孤紫激活胚胎孵裂率影响差异不显著,囊胚发育率分别为29.5%、32.4%、33.9%和41.3%,D7血清能显著提高囊胚发育率（P＜0.05）,且扩张和孵化胚出现较早。添加D0血清体外受精胚胎卵裂率较高,但对囊胚发育率影响差异不显著。说明孤紫激活和体外受精胚胎发育有差异,D7血清对胚胎后期发育有利。受精胚无血清培养3d后添加D7血清,囊胚发育率为42.9%,是较为理想的培养方法。

Effect of Supplementation of Different Estrous Cycle Goat Serum on in Vitro Development of Bovine Embryos

In order to increase the rate of bovine embryo blastocyst development in vitro, different estrous cycle serum of goats was used in culturing embryos. The serums were collected among the estrous cycle.D0,D2,D4 and D7(the day of estrous beginning is D0)goat serum, then sterilired and stored. The result shows the effect of the different serum on the cleavage rate of parthenogenetic embryo is not significant,but the blastocyst rate is 29.5%,32.4%,33.9% and 41.3%, respectively, D7 serum can significantly increase the blastocyst rate and expanded and hatched embryos come earlier.D0 serum can raise the cleavage rate of IVF embryo significantly, but cannot improve the blastocyst formation obviously. It shows that it has the discrepancy between the in vitro developmental competence of the parthenogenetic and IVF embryo,D7 serum was beneficial to later development of bovine embryos. After culturing 3 d without serum, the addition D7 serum can heighten the blastocyst rate of IVF embryo,percentage of blastocyst was 42.9%. This is a better culturing method for culturing embryo in vitro.