| 多菌灵对土壤细菌遗传多样性的影响 |
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| 引用本文： | 肖丽,冯燕燕,赵靓,周川,冶军,危常州,侯振安.多菌灵对土壤细菌遗传多样性的影响[J].新疆农业科学,2011,48(9):1640-1648. |
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| 作者姓名： | 肖丽 冯燕燕 赵靓 周川 冶军 危常州 侯振安 |
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| 作者单位： | 新疆农业科学院吐鲁番农业科学研究所,新疆吐鲁番,838000;石河子大学农学院资环系,新疆石河子,832003 |
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| 基金项目： | 国家科技支撑计划课题“干旱半干旱区土壤农药污染防控关键技术研究与示范”(2007BAc20B04) |
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| 摘 要： | 目的]初步分析多菌灵对土壤细菌群落结构的影响,以期为农药污染的生物监测和土壤环境质量综合评价提供土壤微生物监测指标.方法]采用PCR- DGGE方法研究多菌灵对土壤中细菌群落结构的影响.结果]多菌灵处理土壤的细菌群落种类和结构组成发生了明显变化,细菌群落Shannon - Wiener指数、丰富度和均匀度均有所降低,5.0、10.0和20 mg/kg多菌灵处理的Shannon - Wiener指数均显著低于对照,分别降低了11.47;、19.11;和26.75;.2.5 mg/kg多菌灵处理土壤DGGE图谱上出现了一新增条带,经序列分析为Uncultured Pectobacterium,初步确定为不可培养的菌.结论]施用多菌灵影响了土壤细菌群落的种类和结构组成,改变了土壤细菌的种群数量和结构多样性,导致其多样性降低.
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| 关 键 词： | 多菌灵 土壤 细菌 遗传多样性 PCR-DGGE |
| 收稿时间： | 2011-09-25 |
Effect of Carbendazim on Bacterial Genetic Diversity of Soil |
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| XIAO Li,FENG Yan - yan,ZHAO Jing,ZHOU Chuan YE Jun,WEI Chang - zhou,HOU Zhen - an.Effect of Carbendazim on Bacterial Genetic Diversity of Soil[J].Xinjiang Agricultural Sciences,2011,48(9):1640-1648. |
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| Authors: | XIAO Li FENG Yan - yan ZHAO Jing ZHOU Chuan YE Jun WEI Chang - zhou HOU Zhen - an |
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| Institution: | XIAO Li~1,FENG Yan - yan~2,ZHAO Jing~2,ZHOU Chuan~2 YE Jun~2,WEI Chang - zhou~2,HOU Zhen - an~2 (1.Turpan Research Institute of Agricultural Sciences,Xinjiang Academy of Agricultural Sciences,Turpan 838000,China,2.Department of Resources and Environmental Sciences,College of Agronomy,Shihezi University,Shihezi Xinjiang 832003,China) |
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| Abstract: | Objective]The purpose of the research was to provide comprehensive evaluation monitoring index for the biological monitoring and soil environmental quality of soil which were influenced by pesticide contamination through the preliminary analysis of the effect of carbendazim on bacteria community structure of soil.Method]Effect of carbendazim on bacterial genetic diversity of soil was evaluated with PCR - DGGE method.Result]Variety and structure of bacterial community were changed by adding carbendazim in... |
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| Keywords: | carbendazim soil bacteria genetic diversity PCR - DGGE |
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