红叶石楠组培苗瓶外生根技术研究

以苗龄为40 d的红叶石楠组培继代芽苗为瓶外生根试验材料,采用多因素正交试验设计研究不同栽培基质、不同激素、不同激素浓度处理及处理时间对红叶石楠继代芽苗瓶外生根诱导的影响,继代芽苗扦插前的不同炼苗方式及扦插后施肥方式对芽苗瓶外生根率的影响。结果表明:A3B1C3D2组合(珍珠岩、生根粉ABT 200 mg.L-1浸泡处理30 min)获得59%瓶外生根率,而A3B1C1D2(珍珠岩、ABT 50 mg.L-1浸泡处理30 min)为最理想的组合;芽苗在自然散射光下炼苗15~20 d,生根率可提高到81%;栽后适时追施营养液有利于芽苗的高生长。继代芽苗瓶外生根技术的应用减少组培苗培养工序,促进红叶石楠的组培快繁效率,降低组培苗的生产成本。

Study on rooting techniques of tissue culture buds of Photinia fraseri outside test tubes

Using the proliferation buds aged for 40 days of Photinia fraseri as test materials in multi-factor orthogonal experiment design,the effect of different base soils,different hormones,different concentration treatments of hormones and different treatment time,different acclimating ways and fertilization ways on rooting rate of proliferate buds of Photinia fraseri has been studied.The results showed that 59% rooting rate outside test tube was obtained in the combination of A3B1C3D2(base soil with perlite,being soaked with 200 mg·L-1 ABT rooting powder for 30 minutes);and the combination of A3B1C1D2(base soil with perlite,being soaked with 50 mg·L-1 ABT rooting powder for 30 minutes) is the best for rooting outside test tubes.The rooting rate was increased to 81% when the proliferation buds were cultured for 15～20 days under natural dispersive light;Adding the nutritious liquid benefited high growth of seedlings.Application of rooting techniques of proliferate buds outside test tubes reduces the working procedure of tissue culture seedlings,improving micropropagation efficient of Photinia fraseri and reduced the cost of tissue culture seedlings.