| 用SRAP标记分析黄瓜品种遗传多样性及鉴定品种 |
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| 引用本文： | 李丽,郑晓鹰,柳李旺.用SRAP标记分析黄瓜品种遗传多样性及鉴定品种[J].分子植物育种,2006,4(5):702-708. |
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| 作者姓名： | 李丽 郑晓鹰 柳李旺 |
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| 作者单位： | 北京市农林科学院蔬菜研究中心,北京,100097;南京农业大学园艺学院,南京,210095 |
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| 基金项目： | 上海市科技兴农科技攻关项目 |
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| 摘 要： | 利用相关序列扩增多态性（SRAP）分子标记技术对35份不同类型的黄瓜品种进行了指纹图谱遗传多态性分析，从38对引物组合中筛选出3个多态性高的引物组合，此3对引物扩增得到的图谱可将35份黄瓜品种完全区分开来。依据SRAP指纹图谱聚类分析结果确定了品种之间的遗传距离以及鉴别的难易程度。用SRAP分子标记技术得到了两个黄瓜品种杂交组合F1与亲本的指纹图谱中的鉴别条带，可以用于黄瓜杂交种的纯度检测。
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| 关 键 词： | 黄瓜 指纹图谱 品种鉴定 SRAP |
Analysis of Genetic Diversity and Identification of Cucumber Varieties by SRAP |
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| Li Li,Zheng Xiaoying,Liu Liwang.Analysis of Genetic Diversity and Identification of Cucumber Varieties by SRAP[J].Molecular Plant Breeding,2006,4(5):702-708. |
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| Authors: | Li Li Zheng Xiaoying Liu Liwang |
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| Abstract: | Genetic fingerprint analysis for 35 cucumber varieties from all over countryside revealed by SRAP markers was amplified for identification and register of varieties. In this study 38 primer combinations were screened for 35 varieties. Three primer combinations generated high polymorphic fragments, which can distinguish all of 35 cucumber cultivars. The study demonstrated that molecular markers will provides more efficiency evidence for identification varieties reality. |
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| Keywords: | SRAP |
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