| 水产品肌肉组织中氯霉素残留量检测方法的对比分析 |
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| 引用本文： | 李荣,徐进,艾晓辉,邹世平,伍刚,王鹏. 水产品肌肉组织中氯霉素残留量检测方法的对比分析[J]. 长江大学学报, 2006, 0(11) |
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| 作者姓名： | 李荣 徐进 艾晓辉 邹世平 伍刚 王鹏 |
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| 作者单位： | [1]中国水产科学研究院长江水产研究所 农业部淡水鱼类种质监督检验测试中心 [2]中国水产科学研究院长江水产研究所 [3]农业部淡水鱼类种质监督检验测试中心 [4]湖北省出入境检验检疫局 [5]湖北荆州 [6]湖北武汉43002 |
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| 基金项目： | 科技部重点攻关课题(2002BA906A39) |
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| 摘 要： | 初步比较了酶联免疫法(ELISA)中2种氯霉素试剂盒(荷兰EURO-DIAGNOSTICA试剂盒和德国r-Biopharm试剂盒)检测水产品氯霉素残留量的效果,并对测定水产品氯霉素残留量的气相色谱法(GC-ECD)和液相色谱-质谱法(LC-MS/MS)的技术参数进行了比较分析。结果表明,荷兰EURO-DIAGNOSTICA试剂盒和德国r-Biopharm试剂盒的检测范围分别为0.025~2.0μg.L-1和0.05~4.05μg.L-1,其板内相对标准偏差均小于10%,2种试剂盒检测25个鱼或虾样品的假阳性率分别为12%和20%。LC-MS/MS和GC-ECD的最低检测浓度分别为0.1μg.kg-1和0.27μg.kg-1,当氯霉素添加量为2μg.kg-1时其平均回收率分别为95.2%和70.4%;LC-MS/MS样品前处理较简单,GC-ECD较繁琐。
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| 关 键 词： | 水产品 氯霉素 残留量 试剂盒 检测方法 |
Comparative Analysis of Examination Methods of Chloramphenicol Residue in Aquatic Products |
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| LI Rong,XU Jin,AI Xiao-HuiZHOU Shi-Ping,WU Gang Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Supervision and Testing Center for Freshwater Fish Strain Quality,Ministry of Agriculture,Jingzhou,Hubei ,ChinaWANG Peng. Comparative Analysis of Examination Methods of Chloramphenicol Residue in Aquatic Products[J]. Journal of Yangtze University, 2006, 0(11) |
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| Authors: | LI Rong XU Jin AI Xiao-HuiZHOU Shi-Ping WU Gang Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences Supervision Testing Center for Freshwater Fish Strain Quality Ministry of Agriculture Jingzhou Hubei ChinaWANG Peng |
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| Affiliation: | LI Rong,XU Jin,AI Xiao-HuiZHOU Shi-Ping,WU Gang Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Supervision and Testing Center for Freshwater Fish Strain Quality,Ministry of Agriculture,Jingzhou,Hubei 434000,ChinaWANG Peng |
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| Abstract: | |
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| Keywords: | aquatic product chloramphenicol residue ELISA kit examination method |
| 本文献已被 CNKI 维普 等数据库收录！ |
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